Rabbit anti-Mouse CD23 Monoclonal Antibody for Flow Cytometry - P20693

Core Product Specifications and Parameters

Biological Background: Low Affinity Immunoglobulin Epsilon Fc Receptor (CD23) Function and Localization

• CD23, also known as Fc epsilon RII or Fcer2, is a low-affinity receptor for immunoglobulin E (IgE) and the complement receptor CD21.
• Plays critical roles in regulating IgE production and B-cell differentiation; on B cells, it facilitates IgE-dependent antigen uptake and presentation to T cells.
• On macrophages, CD23 engagement triggers intracellular killing of parasites through activation of the L-arginine-nitric oxide pathway.
• The protein exists as membrane-bound and secreted isoforms via alternative splicing.
• Localizes to the cell membrane; a soluble form is also secreted.
• Post-translationally modified with glycosylation, palmitoylation, and proteoglycan additions; contains disulfide bonds and binds calcium.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody spans amino acids 50‐331 of mouse CD23, covering the extracellular lectin domain, which may recognize both membrane-bound and soluble forms.
• For flow cytometry, consider titrating the antibody to determine optimal signal-to-noise ratio for your specific cell type; use appropriate isotype controls.
• Validate reactivity in the relevant mouse sample system to confirm expected staining patterns.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For allergy and immunology research, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your assay development. Our expertise extends to sourcing rare target raw materials, ensuring reliable supply for your diagnostic needs.