Rabbit anti-Mouse CD226/DNAM-1 Monoclonal Antibody () for Flow Cytometry - Q8K4F0

Core Product Specifications and Parameters

Biological Background: CD226/DNAM-1 Function and Localization

• CD226, also known as DNAM-1, Pta1, and TLiSA1, is a cell surface receptor belonging to the immunoglobulin superfamily.
• Plays a critical role in intercellular adhesion, lymphocyte signaling, cytotoxicity, and lymphokine secretion mediated by cytotoxic T-cells and NK cells. Related references: PMID:19029379 PMID:24658051 PMID:26552706
• Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, it promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill virus-infected or tumor cells. Related references: PMID:30591568
• Mechanistically, phosphorylation by Src kinases (e.g., LYN or FYN) enables binding to adapter GRB2, leading to activation of VAV1, PI3K, PLCG1, ERK, and AKT, as well as calcium fluxes (By similarity).
• Localized to the cell membrane, consistent with its role as a transmembrane receptor.
• Contains an immunoglobulin domain, is glycosylated, and forms disulfide bonds; classified as a phosphoprotein with a signal peptide and transmembrane helix.
• The predicted molecular weight is approximately 38 kDa.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , enabling one-step flow cytometry detection without secondary reagents.
• The immunogen corresponds to the extracellular domain (aa 19-254), suggesting the antibody recognizes an epitope accessible on live cells; confirm optimal staining conditions by titrating on appropriate positive and negative controls.
• Consider using Fc receptor blocking and viability dyes to minimize nonspecific binding and background in flow cytometry assays.
• Validate reactivity in relevant mouse cell lines or primary cells, such as NK cells, CTLs, or T-cell subsets, to ensure specific detection of CD226/DNAM-1.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized one-stop platform providing high-quality IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents, tailored for diagnostic developers, labs, and research institutes. This anti-CD226/DNAM-1 antibody supports research in immuno-oncology, NK cell biology, and immune checkpoint studies, with expert support for rare target sourcing. As your integrated procurement partner, we streamline the transition from concept to clinic with reliable supply chains and technical consultation.