Rabbit anti-Mouse CD226/DNAM-1 Monoclonal Antibody for FC - Q8K4F0

Core Product Specifications and Parameters

Biological Background: CD226/DNAM-1 Function and Localization

• CD226 (DNAM-1/PTA1) is a transmembrane receptor of the immunoglobulin superfamily, primarily expressed on cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells.
• It acts as a costimulatory receptor upon recognition of target cells; binding to ligands PVR/CD155 or NECTIN2/CD112 on infected or tumor cells promotes NK cell and CTL cytotoxicity.
  Related references:
  PMID:30591568
• CD226 mediates intercellular adhesion, lymphocyte signaling, and lymphokine secretion, contributing to immune surveillance.
  Related references:
  PMID:19029379, PMID:24658051, PMID:26552706
• Mechanistically, ligand engagement triggers CD226 phosphorylation by Src-family kinases (LYN/FYN), enabling GRB2 binding and downstream activation of VAV1, PI3K, PLCG1, ERK, AKT, and calcium signaling pathways (By similarity).
• CD226 localizes to the plasma membrane and contains two extracellular Ig-like domains, a single transmembrane helix, and an intracellular region subject to regulatory phosphorylation.
• Post-translational modifications, including glycosylation and disulfide bond formation, are required for proper cell surface expression and function.

Experimental Guidance and Technical Tips

• The antibody was generated against amino acids 19-254 of mouse CD226, corresponding to the extracellular domain; it is expected to recognize the native cell-surface receptor, making it suitable for flow cytometry (FC) applications on viable mouse cells.
• For optimal staining, titrate the antibody over a range of 0.1–10 µg/mL and include appropriate isotype controls and fluorescence-minus-one (FMO) controls to set gates.
• Validate staining on mouse lymphocytes, such as splenocytes or lymph node cells, which express CD226 on NK cells and activated T cells.
• Since CD226 is expressed on cytotoxic lymphocytes, co-staining with lineage markers (e.g., CD3, NK1.1, CD8) can help identify specific cell populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents, to support your diagnostic and research workflows. Our one-stop sourcing bridge simplifies procurement for rare targets like CD226/DNAM-1, ensuring you have the critical reagents needed to advance immunology, immuno-oncology, and infectious disease studies from concept to clinic.