Rabbit anti-Mouse CD22 Monoclonal Antibody for IF-P and FC - P35329

Core Product Specifications and Parameters

Biological Background: CD22 Function and Localization

• CD22 (Siglec-2, BL-CAM) is the most highly expressed sialic acid-binding immunoglobulin-like lectin (siglec) on B-cells and is critical for B-cell differentiation, antigen presentation, and homing to bone marrow. Related references: PMID:7533044 PMID:9016707
• It functions as an inhibitory co-receptor that attenuates B-cell receptor (BCR) signaling by recruiting the tyrosine phosphatase PTPN6/SHP-1 upon phosphorylation of its ITIM motifs by LYN, leading to reduced calcium mobilization and cellular activation. Related references: PMID:9016707
• CD22 binds α2,6-linked sialic acid residues on itself and on other surface molecules such as CD45 and IgM in cis, and also acts as a cell adhesion molecule through trans interactions with ligands on other cells.
• The protein is a type I transmembrane glycoprotein belonging to the siglec family of immunoglobulin-like lectins, characterized by an N-terminal V-set Ig domain and multiple C2-set Ig repeats.
• Multiple isoforms are produced by alternative splicing, with observed molecular weights around 89, 93, and 97 kDa, consistent with the reported full-length 97 kDa form and potential splice variants.
• CD22 is specifically expressed on B-lymphocytes and is localized at the cell membrane, where it shuttles between the cell surface and intracellular compartments.
• Post-translational modifications include N-linked glycosylation, phosphorylation on tyrosine residues within ITIM and ITIM-like motifs, and disulfide bond formation.

Experimental Guidance and Technical Tips

• The immunogen is derived from the extracellular region (aa 22-151) of mouse CD22, making this antibody suitable for detecting surface-exposed CD22 in non-permeabilized cells.
• For flow cytometry (FC), consider titrating the antibody on relevant mouse B-cell lines or primary splenocytes to determine the optimal staining concentration and minimize background.
• In immunofluorescence on paraffin-embedded tissues (IF-P), optimize antigen retrieval conditions, as the epitope may be partially masked by formalin fixation, and include appropriate controls (e.g., CD22-negative cells).
• The conjugated dye is compatible with standard red/orange detection channels; ensure spectral separation if multiplexing with other fluorophores.
• CD22 is a well-validated B-cell marker; this antibody can be used to identify B-lymphocyte populations in mouse tissues, but cross-reactivity with other species has not been tested beyond mouse.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in connecting diagnostic manufacturers, laboratories, and research institutes with premium IVD raw materials—from validated antibody pairs to bulk ancillary reagents. If your work involves CD22-targeted B-cell biology or diagnostic development, we streamline access to optimized monoclonal antibodies like this conjugate, as well as rare target reagents and custom sourcing. Rely on CamelBio for efficient, single-window procurement that supports your journey from concept to clinic.