Rabbit anti-Mouse CD22 Monoclonal Antibody for IF-P and FC - P35329

Core Product Specifications and Parameters

Biological Background: CD22 Function and Localization

• CD22 is a B-cell receptor and the most highly expressed sialic acid-binding immunoglobulin-like lectin (siglec) on B-cells, playing critical roles in differentiation, antigen presentation, and trafficking to bone marrow (PubMed:7533044, PubMed:9016707). Related references: PMID:7533044, PMID:9016707
• It binds α2,6-linked sialic acid residues on surface molecules such as CD22 itself, CD45, and IgM in a cis configuration, and can also act as an adhesion molecule via trans interactions.
• CD22 functions as an inhibitory coreceptor on B-cells, where it negatively regulates B-cell receptor (BCR) signaling by inhibiting calcium mobilization and cellular activation (PubMed:9016707). Related references: PMID:9016707
• Upon BCR engagement, the immunoreceptor tyrosine-based inhibitory motif (ITIM) of CD22 is phosphorylated by Src kinase LYN, leading to recruitment of protein tyrosine phosphatase PTPN6 (SHP-1) and attenuation of BCR signaling.
• If CD22-mediated negative signaling is sufficiently strong, it can induce apoptosis in B-cells.
• CD22 is localized to the cell membrane and is specifically expressed in B-lymphocytes.
• The protein undergoes alternative splicing, and is classified under keywords including glycoprotein, phosphoprotein, disulfide bond, and immunoglobulin domain-containing protein.

Experimental Guidance and Technical Tips

• This -conjugated antibody is designed for direct detection in immunofluorescence (IF-P) and flow cytometry (FC). For IF-P, consider optimizing fixation and permeabilization steps to preserve membrane localization. A recommended starting dilution for FC is typically between 1:50 and 1:200, but end-users should titrate for their specific experimental system.
• The immunogen corresponds to the extracellular domain (amino acids 22-151), targeting the N-terminal region of mouse CD22. This region includes the V-set Ig-like domain involved in sialic acid binding, suggesting the antibody may recognize the receptor in its native conformation.
• Validate specificity using CD22 knockout or knockdown models, if available, to confirm minimal off-target binding in your assays.
• Since CD22 is a B-cell specific marker, ensure the appropriate cell population is present in your samples. For flow cytometry, include appropriate isotype controls and Fc receptor blocking steps.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents tailored for diagnostic development. For CD22-related research, we facilitate access to specialized reagents and rare targets, supporting your workflow from early-stage concept to clinical application. Reach out to our team for customized procurement and technical consultation.