Rabbit anti-Mouse CD21/CD35 Monoclonal Antibody for FC - P19070

Core Product Specifications and Parameters

Biological Background: CD21/CD35 Function and Localization

• CD21/CD35, also known as complement receptor type 2 (CR2) and complement C3d receptor, is a receptor for complement component C3d, HNRNPU, and IFNA1, playing a key role in B-cell antigen recognition and activation.
• Upon binding C3d-coated antigens, CD21/CD35 facilitates antigen uptake and enhances B-cell activation, contributing to the adaptive immune response.
• CD21/CD35 forms the B-cell coreceptor complex with CD19, FCRL5, and CD81, which is crucial for B-cell receptor signaling and lowering the threshold for B-cell activation.
• In addition to its coreceptor function, CD21/CD35 triggers distinct intracellular signaling pathways independently of the BCR and CD19, involving tyrosine kinase SRC-mediated phosphorylation of nucleolin/NCL and downstream activation of AKT and GSK3 kinases.
• CD21/CD35 also acts as a ligand for CD23 (Fc epsilon RII), a low-affinity IgE receptor, participating in the regulation of IgE production.
• The protein is localized to the cell membrane and is specifically expressed on B-lymphocytes.
• Key structural features include disulfide bonds, glycosylation, a signal peptide, sushi repeats, and a transmembrane domain, classifying it as a membrane glycoprotein receptor involved in innate immunity and the complement pathway.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a recombinant fusion protein covering amino acids 21-274, corresponding to an extracellular region of mouse CD21/CD35 that includes the N-terminal sushi domains critical for ligand binding.
• This antibody is conjugated to , a bright and photostable fluorophore suitable for flow cytometry (FC). When designing FC panels, consider potential spectral overlap and include appropriate compensation controls.
• For FC staining of mouse B-lymphocytes, titrate the antibody to determine optimal concentration and validate in the relevant sample system (e.g., splenocytes or lymph node cells) to confirm specificity and minimal background.
• Given the predicted molecular weight of 113 kDa (mature glycoprotein), ensure that sample preparation and detection conditions are appropriate for membrane proteins of this size.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing platform for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. This anti-mouse CD21/CD35 monoclonal antibody, targeting the B-cell complement receptor, is part of our extensive portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Beyond standard offerings, CamelBio excels in sourcing rare-target raw materials, ensuring reliable supply chains for your complement system and B-cell research needs.