Rabbit anti-Mouse CD209b/DC-SIGNR1 Monoclonal Antibody for Flow Cytometry - Q8CJ91

Core Product Specifications and Parameters

Biological Background: CD209b/DC-SIGNR1 Function and Localization

• CD209b, also known as DC-SIGN-related protein 1 (DC-SIGNR1), is a C-type lectin receptor primarily expressed on dendritic cells.
• Functions as a pathogen-recognition receptor, binding high mannose N-linked oligosaccharides in a calcium-dependent manner.
• Mediates endocytosis of pathogens for lysosomal degradation and serves as a receptor for ICAM3.
• Localized to the cell membrane as a single-pass type II transmembrane protein.
• Expressed in skin, spleen, and lung, particularly in dendritic cells within extrafollicular paracortical areas.
• Undergoes alternative splicing, yielding isoforms with molecular weights ranging from 16 to 37 kDa.
• Contains a carbohydrate recognition domain (CRD), transmembrane helix, and signal-anchor; classified as a glycoprotein with disulfide bonds.
• Associated with keywords: endocytosis, mannose-binding, metal-binding, and alternative splicing.

Experimental Guidance and Technical Tips

• The antibody is directly conjugated to , making it ready-to-use for flow cytometry; titrate to determine the optimal working concentration for your cell model.
• The immunogen (aa 76–325) covers the extracellular region, including the carbohydrate recognition domain; ensure that target accessibility is preserved under your staining conditions.
• Given the multiple reported molecular weights due to alternative splicing and glycosylation, confirm the specific isoform(s) present in your sample system by western blot if needed.
• Validate antibody specificity using appropriate mouse tissue controls (e.g., spleen, skin) known to express CD209b.
• For intracellular staining, consider mild permeabilization protocols, although the native protein is surface-exposed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, from validated monoclonal antibodies like this CD209b/DC-SIGNR1 clone to bulk ancillary reagents for diagnostic development. We support your dendritic cell and pathogen recognition research with flexible sourcing solutions, including rare target procurement and large-scale supply. Partner with CamelBio to streamline your journey from concept to clinic.