Rabbit anti-Mouse CD205/DEC-205 Monoclonal Antibody for Flow Cytometry - Q60767

Core Product Specifications and Parameters

Biological Background: CD205/DEC-205 Function and Localization

• CD205 (Lymphocyte antigen 75, DEC-205) is a type I transmembrane glycoprotein and endocytic receptor belonging to the C-type lectin superfamily.
• Acts as an antigen-uptake receptor on dendritic cells, routing extracellular antigens to specialized processing compartments for presentation to T cells.
• By similarity, engagement of CD205 may lead to reduced proliferation of B lymphocytes, suggesting a regulatory role in humoral immunity.
• Structurally, it contains multiple C-type lectin-like domains, a transmembrane helix, and a cytoplasmic tail with endocytosis motifs; its predicted molecular weight is approximately 197 kDa.
• Primarily expressed on dendritic cells, thymic epithelial cells, and in lymph nodes, consistent with its role in antigen handling and immune surveillance.
• Post-translational modifications include disulfide bonds, glycosylation, and phosphorylation, which likely influence ligand binding and trafficking.

Experimental Guidance and Technical Tips

• This antibody is designed for flow cytometry; conjugate brightness () is compatible with common red laser detectors.
• The immunogen covers amino acids 216-490 of the mouse CD205 extracellular region, which includes multiple lectin domains; consider this when interpreting binding patterns in mutants or isoforms.
• Validate antibody staining using appropriate mouse dendritic cell lines (e.g., DC2.4) or primary splenic dendritic cells; include isotype controls to assess background.
• For best results, titrate the antibody to determine optimal concentration for your application and instrument settings.
• CD205 is rapidly internalized upon ligand binding; for surface staining, fix cells promptly or perform staining at 4°C to minimize internalization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement of IVD raw materials for diagnostic developers and research labs. For immunology projects targeting CD205, we can source validated antibody pairs, recombinant proteins, and ancillary reagents to accelerate your assay development. Our integrated supply network and technical support help you move from concept to clinic with confidence.