Rabbit anti-Mouse CD205/DEC-205 Monoclonal Antibody for FC - Q60767

Core Product Specifications and Parameters

Biological Background: CD205/DEC-205 Function and Localization

• Lymphocyte antigen 75, also known as DEC-205 or CD205, is encoded by the Ly75 gene in mouse.
• It functions as an endocytic receptor that directs captured antigens to specialized antigen-processing compartments, playing a role in immune responses.
• In B lymphocytes, CD205 engagement has been shown to reduce proliferation, indicating a regulatory role (reported by similarity).
• The protein is a single-pass type I transmembrane glycoprotein located on the cell membrane.
• CD205 is predominantly expressed in dendritic cells, thymic epithelial cells, and lymph nodes, highlighting its role in antigen presentation.
• Contains C-type lectin domains, fibronectin type II domains, and multiple disulfide bonds, characteristic of the macrophage mannose receptor family.
• Post-translational modifications include glycosylation and phosphorylation, which may modulate its function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 216-490, an extracellular region; thus, this antibody is suitable for detecting surface CD205 by flow cytometry.
• For flow cytometry, use standard staining protocols with appropriate isotype controls and consider blocking Fc receptors to minimize nonspecific binding.
• Validate the antibody in mouse splenocyte or lymph node cell suspensions, where CD205 is expressed on dendritic cell subsets.
• Titrate the antibody to determine optimal concentration for your specific assay, as performance may vary with cell type and staining conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies, recombinant proteins, and ancillary reagents, serving diagnostic manufacturers, researchers, and clinical laboratories worldwide. This anti-mouse CD205/DEC-205 antibody supports immunological and dendritic cell research, and CamelBio can further assist with sourcing partner antibodies, bulk reagent supplies, and custom development to streamline your workflow from concept to clinic.