Rabbit anti-Mouse CD205/DEC-205 Monoclonal Antibody () for FC - Q60767

Core Product Specifications and Parameters

Biological Background: CD205/DEC-205 Function and Localization

• Lymphocyte antigen 75 (Ly75), also known as CD205 or DEC-205, is a ~197 kDa type I transmembrane glycoprotein and member of the mannose receptor family.
• It functions as an endocytic receptor that directs captured antigens from the extracellular space to specialized antigen-processing compartments, and causes reduced proliferation of B lymphocytes.
• Subcellularly, CD205/DEC-205 localizes to the membrane.
• It is expressed in dendritic cells, thymic epithelial cells, and lymph nodes.
• The protein contains multiple C-type lectin-like domains, a fibronectin type II domain, and a cysteine-rich domain, facilitating carbohydrate recognition and antigen uptake.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation.

Experimental Guidance and Technical Tips

• This antibody was generated using a recombinant fusion protein covering amino acids 216-490 of mouse CD205, and is conjugated to the bright, photostable fluorophore, optimized for flow cytometry.
• For flow cytometry, titrate the antibody to determine the optimal staining concentration for your cell population, and include appropriate isotype controls and viability dyes.
• Given its expression on dendritic cells, thymic epithelial cells, and lymph node cells, prepare single-cell suspensions from relevant tissues and consider enrichment strategies to enhance detection.
• The target protein is a large membrane protein (~197 kDa); for intracellular staining, validate permeabilization conditions accordingly.
• Fluorescence signal may be affected by antibody internalization if working at 37°C; consider staining at 4°C or fixing cells prior to staining if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, streamlining access to high-quality research and diagnostic reagents like this CD205/DEC-205 monoclonal antibody for dendritic cell and immunophenotyping studies. We supply validated antibody pairs, optimized monoclonals, and bulk ancillary reagents, along with rare target sourcing services to accelerate your assay development from concept to clinic.