Rabbit anti-Mouse CD200/OX2 Monoclonal Antibody for FC - O54901

Core Product Specifications and Parameters

Biological Background: CD200/OX2 Function and Localization

• CD200, also known as OX-2 membrane glycoprotein or MRC OX-2 antigen, is a cell surface glycoprotein belonging to the immunoglobulin superfamily.
• It functions as a costimulatory molecule for T-cell proliferation, likely through interaction with its receptor.
• CD200 may regulate myeloid cell activity in various tissues, contributing to immune homeostasis.
• The protein is localized to the cell membrane and contains a single-pass transmembrane domain.
• Key structural features include an extracellular immunoglobulin-like domain, a signal peptide, and a disulfide bond within the Ig domain.
• It undergoes N-linked glycosylation, and its predicted mature molecular weight is approximately 31 kDa (consistent with the product's reported 31 kDa).
• The CD200 gene (Mox2) is conserved across species, with the mouse protein sharing homology with the human CD200 (Gene ID 4345).

Experimental Guidance and Technical Tips

• The antibody is directly conjugated to , making it ready-to-use for flow cytometry without the need for secondary detection reagents.
• The immunogen (amino acids 31-232) corresponds to the extracellular domain of mouse CD200, suggesting the antibody likely recognizes the native form on the cell surface; validation in the relevant sample system is recommended.
• For flow cytometry, titrate the antibody to determine the optimal concentration, and include appropriate isotype controls and viability staining to ensure specificity.
• While this product is validated for flow cytometry, its suitability for other applications (e.g., IHC, WB) has not been established; if other applications are required, additional testing and optimization should be performed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials and technical support. This CD200/OX2 monoclonal antibody is ideal for immune cell profiling and biomarker studies, and we can also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development. Partner with us to streamline your procurement from concept to clinic.