Rabbit anti-Mouse CD2 Monoclonal Antibody for Flow Cytometry - P08920

Core Product Specifications and Parameters

Biological Background: CD2 Function and Localization

• CD2 is a T-cell surface glycoprotein belonging to the immunoglobulin superfamily, also known as LFA-2, Ly-37, or T11/Leu-5.
• It mediates adhesion between T-cells and antigen-presenting cells or target cells by binding to CD58 (LFA-3) and CD48/BCM1.
• The cytoplasmic domain of CD2 is involved in T-cell activation and signaling pathways upon ligand engagement.
• CD2 is located on the cell membrane and contains a signal peptide, two immunoglobulin-like domains, a single transmembrane helix, and a cytoplasmic tail.
• Expression of CD2 is detected primarily in the thymus and spleen, consistent with its role in immune cell interactions.
• Post-translational modifications include N-linked glycosylation and a conserved disulfide bond in the extracellular domain, which are critical for proper folding and function.

Experimental Guidance and Technical Tips

• Titrate the antibody to determine the optimal concentration for your specific flow cytometry assay and sample type.
• Use appropriate isotype controls and fluorescence minus one (FMO) controls to accurately set gates and assess background.
• Because is a bright fluorophore, carefully design your multicolor panel to minimize spectral overlap and compensation issues.
• Validate staining in known positive tissues or cell lines (e.g., mouse thymocytes or splenocytes) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for sourcing high-quality IVD raw materials, offering a comprehensive portfolio including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For projects targeting CD2 in immunological research, CamelBio streamlines procurement and provides expert technical support from concept to clinic. Let us bridge your sourcing needs with reliable solutions.