Rabbit anti-Mouse CD2 Monoclonal Antibody for FC - P08920

Core Product Specifications and Parameters

Biological Background: CD2 Function and Localization

• CD2 is a T-cell surface antigen belonging to the immunoglobulin superfamily, also known as LFA-2, Ly37, and T11/Leu-5.
• It mediates adhesion between T-cells and antigen-presenting cells or target cells through interaction with ligands CD58 (LFA-3) and CD48/BCM1.
• CD2 is implicated in T-cell activation and co-stimulation; its cytoplasmic domain participates in signaling pathways that enhance T-cell responses.
• The protein is localized on the cell membrane, with a single-pass transmembrane domain.
• It is expressed in lymphoid tissues, particularly thymus and spleen.
• Post-translational modifications include glycosylation and disulfide bond formation.
• Contains immunoglobulin-like domains and a signal peptide, consistent with its role as a cell surface receptor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 23-203), making this antibody suitable for surface staining in live cells without permeabilization.
• Titrate the antibody to determine the optimal concentration for your specific flow cytometry protocol.
• As the antibody is pre-conjugated to , no secondary detection is needed; ensure proper compensation controls when using multiple fluorophores.
• Include an appropriate isotype control to assess background staining.
• Validate specificity using CD2-deficient mouse models or cell lines to confirm target recognition.
• Protect the antibody from prolonged light exposure to maintain fluorophore stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, offering a curated selection of validated antibodies, bulk reagents, and specialized sourcing services for immunoassay development. For researchers studying T-cell biology or infectious immunity, we provide optimized monoclonal antibodies like this anti-CD2 clone, backed by reliable supply chains and technical support. Our integrated platform helps streamline your diagnostic projects from initial research to clinical application.