Rabbit anti-Mouse CD2 mAb for FC - P08920

Core Product Specifications and Parameters

Biological Background: CD2 Function and Localization

• CD2 is a T-cell surface antigen belonging to the immunoglobulin superfamily, also known as LFA-2, Ly-37, and T11/Leu-5.
• It mediates cell adhesion by binding to ligands CD58 (LFA-3) and CD48/BCM1, facilitating interactions between T-cells and antigen-presenting or target cells.
• The cytoplasmic domain of CD2 is involved in T-cell activation and signal transduction, contributing to the triggering of T-cell responses.
• CD2 is localized to the cell membrane, with a single-pass transmembrane domain and an extracellular region containing immunoglobulin-like domains.
• It is post-translationally modified with N-linked glycosylation and contains disulfide bonds, consistent with its role in immune recognition.
• CD2 is expressed in lymphoid tissues, notably the thymus and spleen, where it plays a role in T-cell development and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 23-203) of mouse CD2, making this antibody suitable for surface staining in flow cytometry without permeabilization.
• For optimal results, consider titrating the antibody to determine the best staining concentration in your experimental system.
• Use appropriate Fc blocking reagents and isotype controls to reduce non-specific binding, especially when analyzing T-cell populations from spleen or thymus.
• Validate the antibody’s performance in your specific sample type and fixation conditions, as epitope accessibility may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers integrated access to IVD raw materials for immunology and T-cell biology research, including this anti-mouse CD2 antibody. We supply validated monoclonal and polyclonal antibodies, optimized antibody pairs, and bulk ancillary reagents to streamline your diagnostic development. For rare targets or custom sourcing needs, our team bridges the gap from concept to clinic with reliable procurement solutions.