Rabbit anti-Mouse CD19 Monoclonal Antibody for Flow Cytometry - P25918

Core Product Specifications and Parameters

Biological Background: CD19 Function and Localization

• Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes, decreasing the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (By similarity).
• Activates signaling pathways leading to phosphatidylinositol 3-kinase activation and mobilization of intracellular Ca2+ stores. Related references: PMID:12387743 PMID:20101619 PMID:9382888
• Is not required for early steps during B cell differentiation in the bone marrow. Related references: PMID:7542548 PMID:7543183 PMID:9317126
• Required for normal differentiation of B-1 cells. Related references: PMID:12387743 PMID:7542548 PMID:7543183
• Required for normal B cell differentiation and proliferation in response to antigen challenges. Related references: PMID:12387743 PMID:7542548 PMID:9317126
• Required for normal levels of serum immunoglobulins and for production of high-affinity antibodies in response to antigen challenge. Related references: PMID:12387743 PMID:7542548 PMID:7543183
• Subcellular location: Cell membrane; membrane raft.
• Tissue specificity: Detected on B cells in spleen, bone marrow, thymus and lymph nodes, and on peripheral blood lymphocytes (at protein level). Related references: PMID:12387743 PMID:20101619 PMID:7542548 PMID:7543183
• Post-translational modifications and keywords: Glycoprotein, disulfide bond, phosphoprotein, immunoglobulin domain, adaptive immunity, cell membrane.

Experimental Guidance and Technical Tips

• The antibody was generated using recombinant full-length CD19 protein as immunogen, which likely preserves native epitopes; consider validating reactivity with your cell system.
• Recommended application is flow cytometry. Titrate the antibody in your specific experimental system to determine optimal staining concentration.
• CD19 is expressed on B cells; use appropriate B-cell markers (e.g., B220) and isotype controls to confirm specificity.
• Ensure proper handling: store at recommended temperature and protect from light to maintain fluorescence.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner for diagnostic manufacturers, research labs, and institutes, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target custom sourcing. This mouse CD19 monoclonal antibody is ideal for flow cytometric immunophenotyping in B-cell research and assay development. Contact us to explore how our comprehensive supply capabilities can support your IVD projects from concept to clinic.