Rabbit anti-Mouse CD19 Monoclonal Antibody for Flow Cytometry - P25918

Core Product Specifications and Parameters

Biological Background: CD19 Function and Localization

• CD19 is a B-lymphocyte-specific cell surface antigen that functions as a coreceptor for the B-cell antigen receptor complex (BCR). It reduces the signaling threshold required for B-cell activation in response to antigens (By similarity).
• Upon BCR engagement, CD19 activates downstream phosphatidylinositol 3-kinase (PI3K) signaling and mediates the mobilization of intracellular calcium stores. Related references: PMID:12387743 PMID:20101619 PMID:9382888
• CD19 is not essential for early B cell development in the bone marrow but is required for the normal differentiation of B-1 cells and for effective B cell responses to antigen challenges. Related references: PMID:12387743 PMID:7542548 PMID:7543183 PMID:9317126
• It is critical for normal serum immunoglobulin levels and the production of high-affinity antibodies following antigen exposure. Related references: PMID:12387743 PMID:7542548 PMID:7543183
• CD19 localizes to the cell membrane and associates with membrane rafts, which are important for signal transduction (By similarity).
• Tissue expression analysis shows CD19 is detected on B cells in the spleen, bone marrow, thymus, lymph nodes, and peripheral blood lymphocytes. Related references: PMID:12387743 PMID:20101619 PMID:7542548 PMID:7543183
• Key molecular features of CD19 include immunoglobulin-like extracellular domains, a transmembrane helix, and conserved phosphorylation motifs, aligning with its role as a signaling adaptor in adaptive immunity (UniProt P25918).

Experimental Guidance and Technical Tips

• This antibody was generated using a recombinant mouse CD19 immunogen and is validated for flow cytometry. For optimal performance in FC, consider titrating the antibody and using appropriate isotype controls.
• Given the expression of CD19 on B cells, the antibody is suitable for identifying and gating B-cell populations in lymphoid tissues such as spleen, bone marrow, and lymph nodes.
• To maintain surface antigen integrity, process samples quickly and use cold buffers during cell preparation and staining.
• As with any conjugate, protect the antibody from light and validate specificity in the relevant sample system prior to use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw material procurement by offering a comprehensive portfolio of validated antibody pairs, monoclonal antibodies, and ancillary reagents. For research involving CD19 and B-cell immunophenotyping, we provide bulk sourcing options matched to diagnostic development needs. Contact us for customized raw-material solutions and technical support.