Rabbit anti-Mouse CD19 Monoclonal Antibody for FC - P25918

Core Product Specifications and Parameters

Biological Background: Mouse CD19 Function and Localization

• CD19 acts as a coreceptor for the B-cell antigen receptor (BCR), decreasing the activation threshold and triggering downstream signaling via PI3K and intracellular calcium mobilization. Related references: PMID:12387743 PMID:20101619 PMID:9382888
• CD19 is not required for the early stages of B cell differentiation in the bone marrow. Related references: PMID:7542548 PMID:7543183 PMID:9317126
• CD19 is essential for the normal differentiation of B-1 cells. Related references: PMID:12387743 PMID:7542548 PMID:7543183
• CD19 is required for antigen-driven B cell differentiation and proliferation, and for the production of high-affinity antibodies and maintenance of normal serum immunoglobulin levels. Related references: PMID:12387743 PMID:7542548 PMID:7543183 PMID:9317126
• CD19 localizes to the cell membrane and membrane rafts, positioning it for efficient signal transduction.
• CD19 is predominantly expressed on B cells in the spleen, bone marrow, thymus, lymph nodes, and peripheral blood. Related references: PMID:12387743 PMID:20101619 PMID:7542548 PMID:7543183
• CD19 is a glycoprotein and phosphoprotein belonging to the immunoglobulin superfamily, characterized by disulfide bonds and immunoglobulin-like domains.

Experimental Guidance and Technical Tips

• This antibody is supplied as an conjugate, ready for direct flow cytometry analysis on mouse samples.
• For applications beyond flow cytometry, consider using an unconjugated primary antibody format or an alternative conjugate.
• Perform a titration experiment to determine the optimal working concentration for your specific experimental setup and sample type.
• Include appropriate isotype control antibodies and Fc receptor blocking reagents to ensure specific staining.
• Validate the antibody performance in your mouse B-cell model system, considering the natural expression pattern of CD19.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop partner for IVD raw materials, offering validated antibody pairs, recombinant proteins, and bulk ancillary reagents. This anti-mouse CD19 monoclonal antibody supports B-cell immunophenotyping studies and diagnostic kit development. With expertise in rare target sourcing and supply chain reliability, CamelBio streamlines procurement from concept to clinic.