Rabbit anti-Mouse CD19 Monoclonal Antibody for FC - P25918

Core Product Specifications and Parameters

Biological Background: CD19 Function and Localization

• CD19 is a B-lymphocyte surface antigen that functions as a coreceptor for the B-cell antigen receptor complex (BCR), lowering the threshold for B-cell activation.
• Upon BCR engagement, CD19 activates phosphatidylinositol 3-kinase (PI3K) and mobilizes intracellular Ca2+ stores, critical for downstream signaling. Related references: PMID:12387743 PMID:20101619 PMID:9382888
• CD19 is required for normal differentiation of B-1 cells, a subset of B cells involved in innate-like immunity. Related references: PMID:12387743 PMID:7542548 PMID:7543183
• It is essential for normal B cell differentiation and proliferation in response to antigen challenges, and for production of high-affinity antibodies. Related references: PMID:12387743 PMID:7542548 PMID:9317126
• CD19 localizes to the cell membrane and membrane rafts, where it interacts with other signaling molecules.
• In mice, CD19 is detected on B cells in the spleen, bone marrow, thymus, lymph nodes, and peripheral blood lymphocytes. Related references: PMID:12387743 PMID:20101619 PMID:7542548 PMID:7543183
• Structurally, CD19 contains immunoglobulin-like domains, a single transmembrane helix, and a cytoplasmic tail that undergoes phosphorylation. It is a glycoprotein with disulfide bonds.

Experimental Guidance and Technical Tips

• This antibody is designed for flow cytometry (FC) and is directly conjugated to the bright fluorophore , eliminating the need for a secondary antibody.
• The immunogen spans the extracellular domain (amino acids 19–287), targeting a region likely accessible on live cells. Consider surface staining protocols for optimal detection.
• Titrate the antibody to determine the optimal working concentration for your specific mouse B-cell sample.
• Include appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials for B-cell and immunology research, including validated antibody pairs, conjugated monoclonal antibodies, and ancillary reagents. The anti-CD19 antibody targets a critical lymphocyte surface marker, supporting diagnostic and research applications in immune profiling and hematological disorders. As your one-stop sourcing partner, we help streamline procurement from early-stage development through clinical validation.