Rabbit anti-Mouse CD19 Monoclonal Antibody for FC - P25918

Core Product Specifications and Parameters

Biological Background: CD19 Function and Localization

• CD19 is a B-lymphocyte surface antigen that functions as a coreceptor for the B-cell antigen receptor complex (BCR), lowering the threshold for activation of downstream signaling pathways and B-cell responses to antigens.
• Upon BCR engagement, CD19 activates phosphatidylinositol 3-kinase and mobilizes intracellular Ca²⁺ stores, amplifying signaling cascades. Related references: PMID:12387743, PMID:20101619, PMID:9382888
• CD19 is not required for early B-cell differentiation in bone marrow but is essential for normal B-1 cell development and for B-cell differentiation and proliferation in response to antigen challenges. Related references: PMID:7542548, PMID:7543183, PMID:9317126
• The protein is required for normal serum immunoglobulin levels and for production of high-affinity antibodies following antigen challenge. Related references: PMID:12387743, PMID:7542548, PMID:7543183
• CD19 is localized to the cell membrane and membrane rafts, consistent with its role in signal transduction.
• Its expression is detected on B cells in spleen, bone marrow, thymus, lymph nodes, and peripheral blood lymphocytes.
• The protein is a glycoprotein with immunoglobulin domains, disulfide bonds, and phosphorylation sites, indicating complex post-translational modification and structural motifs important for its function.

Experimental Guidance and Technical Tips

• is a bright fluorophore; ensure proper compensation and use appropriate controls in multicolor flow cytometry panels.
• Validate the antibody in the relevant mouse B-cell sample system, such as splenocytes, lymph node cells, or peripheral blood lymphocytes.
• The immunogen corresponds to amino acids 19–287, which is the extracellular region of CD19, suggesting the antibody likely recognizes an extracellular epitope suitable for live-cell staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to provide one-stop access to IVD raw materials, including validated monoclonal antibodies for immunology research. For B-cell and lymphocyte studies, we offer bulk quantities of anti-CD19 reagents and can assist in sourcing rare-target antibodies. Our integrated technical and consulting services support your workflow from concept to clinic.