Rabbit anti-Mouse CD19 mAb for FC - P25918

Core Product Specifications and Parameters

Biological Background: B-lymphocyte antigen CD19 Function and Localization

• CD19, also known as differentiation antigen CD19, is a B-lymphocyte surface antigen that functions as a coreceptor for the B-cell antigen receptor complex (BCR).
• It decreases the threshold for activation of downstream signaling pathways, including phosphatidylinositol 3-kinase activation and mobilization of intracellular Ca²⁺ stores. Related references: PMID:12387743, PMID:20101619, PMID:9382888
• CD19 is not required for early B cell differentiation in the bone marrow but is essential for normal differentiation of B-1 cells. Related references: PMID:7542548, PMID:7543183, PMID:9317126, PMID:12387743
• It is required for normal B cell differentiation and proliferation in response to antigen challenges. Related references: PMID:12387743, PMID:7542548, PMID:9317126
• CD19 is necessary for normal serum immunoglobulin levels and production of high-affinity antibodies upon antigen challenge. Related references: PMID:12387743, PMID:7542548, PMID:7543183
• It localizes to the cell membrane and membrane rafts, consistent with its transmembrane signaling role.
• Tissue specificity: CD19 is detected on B cells in the spleen, bone marrow, thymus, lymph nodes, and on peripheral blood lymphocytes. Related references: PMID:12387743, PMID:20101619, PMID:7542548, PMID:7543183
• Additional keywords from UniProt: CD19 contains immunoglobulin domains, disulfide bonds, glycosylation sites, and is a phosphoprotein, underscoring its role as a signaling receptor.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to a fluorophore; no secondary detection step is required for flow cytometry applications.
• Titrate the antibody concentration to optimize staining index and minimize background in your specific mouse B-cell sample system.
• Consider using an Fc receptor blocking step to reduce non-specific binding when staining mouse lymphoid tissues.
• Include appropriate isotype control and viability dye in your panel to discriminate live B-cell populations from debris and dead cells.
• Validate the staining pattern using known CD19-positive (e.g., splenic B cells) and CD19-negative (e.g., T cells) controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with integrated access to IVD raw materials, from validated antibody pairs and conjugates to bulk ancillary reagents, accelerating B-cell immunophenotyping and leukemia/lymphoma assay development. For projects requiring rare target raw-material sourcing or technical consulting from concept to clinic, our team offers end-to-end support.