Rabbit anti-Mouse CD161c/NK1.1 Monoclonal Antibody for Flow Cytometry - P27814

Core Product Specifications and Parameters

Biological Background: CD161c/NK1.1 (Klrb1c) Function and Localization

• CD161c/NK1.1 (gene Klrb1c) is a mouse killer cell lectin-like receptor subfamily B member 1C, also known as Ly-55c, NKR-P1C, or NKRP1.9.
• It functions as a stimulatory receptor on natural killer (NK) cells; cross-linking of CD161c/NK1.1 triggers Ca²⁺ mobilization and interferon-gamma production, enhancing cytotoxicity.
• CD161c/NK1.1 belongs to the NKRP1 family of C-type lectin-like receptors within the larger natural killer gene complex.
• The protein is a single-pass transmembrane glycoprotein, containing an extracellular C-type lectin domain, a signal-anchor, and an intracellular domain likely involved in signaling.
• It is predominantly expressed on mouse NK cells and serves as a defining cell surface marker for NK cells in certain mouse strains.
• Post-translational modifications include disulfide bond formation and N-linked glycosylation, contributing to its stability and surface expression.
• The receptor undergoes alternative splicing, generating isoforms that may modulate NK cell activity.

Experimental Guidance and Technical Tips

• The antibody is validated for flow cytometry; consider using appropriate isotype controls and Fc receptor blocking to minimize background binding.
• The immunogen corresponds to the extracellular domain (amino acids 67–223) of mouse CD161c/NK1.1, which is likely to recognize the native surface receptor.
• For optimal detection, titrate the antibody in your specific assay system and validate in relevant mouse NK cell populations (e.g., spleen, blood).
• Note that CD161c/NK1.1 expression is strain-specific; ensure your mouse strain (e.g., C57BL/6) expresses the antigen before experimentation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to critical raw materials and technical services from concept to clinic. This CD161c/NK1.1 antibody supports immunology and NK cell research, and is part of our broad portfolio of validated monoclonal antibodies, antibody pairs, and ancillary reagents. From bulk antibodies to rare-target sourcing, CamelBio is your integrated partner for IVD development.