Rabbit anti-Mouse CD161c/NK1.1 Monoclonal Antibody for Flow Cytometry - P27814

Core Product Specifications and Parameters

Biological Background: CD161c/NK1.1 Function and Localization

• Klrb1c encodes killer cell lectin-like receptor subfamily B member 1C, a type II transmembrane glycoprotein of the C-type lectin superfamily commonly referred to as CD161c, NK1.1, or NKR-P1C.
• The receptor functions as an activating molecule on natural killer (NK) cells; cross-linking stimulates NK cell cytotoxicity and induces intracellular Ca²⁺ mobilization and interferon-gamma (IFN-γ) secretion.
• Klrb1c belongs to the Klrb (NKRP1) gene cluster, which includes several highly related genes encoding NK cell receptors with distinct regulatory roles.
• The protein is localized to the plasma membrane, anchored by an N-terminal signal-anchor transmembrane helix, and its extracellular region contains a single C-type lectin-like domain responsible for ligand recognition.
• Post-translational modifications include N-linked glycosylation and formation of disulfide bonds, which are important for proper folding and stability of the extracellular domain.
• Expression of Klrb1c is predominantly restricted to natural killer cells, consistent with its role in innate immune surveillance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 67-223), suggesting the antibody recognizes an epitope accessible on live, unfixed cells, making it suitable for surface staining by flow cytometry.
• For flow cytometry, consider titrating the antibody to determine the optimal concentration for your cell type and experimental conditions.
• As an conjugate, avoid prolonged exposure to light and use appropriate compensation controls in multicolor panels.
• Expression of NK1.1 is known to vary among mouse strains; confirm reactivity with your specific model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw-material procurement by offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our portfolio includes antibodies targeting immune cell surface markers such as CD161c/NK1.1, ideal for flow cytometry-based immunophenotyping in immunology research and diagnostic assay development. For rare targets or custom antibody projects, CamelBio provides expert sourcing and technical support.