Rabbit anti-Mouse CD150/SLAM Monoclonal Antibody for Flow Cytometry - Q9QUM4

Core Product Specifications and Parameters

Biological Background: CD150/SLAM Function and Localization

• CD150, also known as signaling lymphocytic activation molecule (SLAM) and SLAMF1, is a self-ligand receptor of the immunoglobulin superfamily that modulates activation and differentiation in a broad range of immune cells.
• It mediates IL-2-independent proliferation of activated T‑cells and induces IFN‑γ production, driving key adaptative immune responses. Related references: PMID:12351401 PMID:9126961
• Downstream signaling involves SH2D1A/SAP and FYN kinase; in T‑cells, SLAMF1 also engages the PRKCQ–BCL10–NFKB1 axis to enhance T‑cell activation and Th2 cytokine production. Related references: PMID:11477403 PMID:15539155 PMID:16847311
• In B‑cells, SLAMF1 activates ERK and Akt pathways independent of SAP, implicating SYK and INPP5D, and together with CD84 and SLAMF6 it may negatively regulate the humoral immune response. Related references: PMID:15315965 PMID:25926831
• It is required for IL‑4 production by germinal center T follicular helper cells and controls the transition and differentiation of thymic NKT cells toward a regulatory NKT2 phenotype. Related references: PMID:20525889 PMID:18031695 PMID:18606638
• In macrophages, SLAMF1 down‑regulates LPS‑induced IL‑12, TNF, and nitric oxide, and additionally functions as an innate sensor for Gram‑negative bacteria by recognizing OmpC/OmpF, promoting phagosome maturation and NOX2 recruitment. Related references: PMID:15123745 PMID:20818396 PMID:22493499
• The protein is a single‑pass type I membrane glycoprotein with an extracellular region containing immunoglobulin‑like domains; it is expressed on the cell surface of immune cells where it engages in homotypic interactions.
• Key post‑translational modifications include disulfide bonds, glycosylation, and phosphorylation on cytoplasmic tyrosines following ligand engagement.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 25‑242) of mouse CD150, encompassing the ligand‑binding Ig‑like domains, making this monoclonal antibody well‑suited for detecting surface‑expressed CD150 by flow cytometry.
• Direct conjugation eliminates the need for a secondary detection reagent; titrate the antibody (e.g., 0.5–5 µg/mL) on your target cells and include appropriate isotype and fluorescence minus one (FMO) controls.
• For optimal staining, use a standard flow cytometry staining buffer (PBS with BSA or FBS) and acquire data on a cytometer equipped with a blue laser (488 nm) and FITC/GFP channel.
• Confirm reactivity in mouse splenocytes, lymph node cells, or cultured immune cell lines; if working with activated T‑cells, note that CD150 expression can be up‑regulated upon stimulation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a streamlined, one‑stop access to IVD raw materials, ensuring traceable and consistent supply for every stage of product development. This anti‑CD150/SLAM antibody exemplifies our portfolio for immunology and immune‑oncology research. Alongside single‑reagent sourcing, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare‑target raw materials—supported by technical consulting to help you move from concept to clinic with confidence.