Rabbit anti-Mouse CD150/SLAM mAb - Q9QUM4

Core Product Specifications and Parameters

Biological Background: CD150/SLAM Function and Localization

• CD150/SLAM (SLAMF1) is a self-ligand receptor of the signaling lymphocytic activation molecule family, modulating immune cell activation and differentiation through homo- or heterotypic cell-cell interactions.
• Two modes of SLAMF1 signaling exist: one depending on SH2D1A/SAP (and perhaps SH2D1B) and another involving PTPN11-dependent signal transduction.
• In T-cells, SLAMF1 triggers IL-2-independent proliferation and IFN-gamma production, with downstream signaling involving INPP5D, DOK1/2, PRKCQ, BCL10, and NFKB1. Related references: PMID:12351401, PMID:9126961, PMID:11477403, PMID:15539155, PMID:16847311
• SLAMF1 enhances TCR-induced IL-4 secretion by CD4+ T cells and is required for IL-4 production by germinal center T follicular helper cells, while inhibiting IFN-gamma in CD4-/CD8- T cells. Related references: PMID:15123745, PMID:11477403, PMID:20525889, PMID:16528012
• Together with SLAMF6, SLAMF1 controls the transition between positive selection and expansion/differentiation of thymic NKT cells, and regulates peripheral iNKT cell differentiation toward a regulatory NKT2 type. Related references: PMID:18031695, PMID:18606638
• In B-cells, SLAMF1 activates ERK and Akt signaling pathways, and together with SLAMF5 and SLAMF6 may negatively regulate humoral immune responses; in macrophages, it down-regulates IL-12, TNF, and nitric oxide in response to LPS. Related references: PMID:15315965, PMID:15123745, PMID:25926831
• Involved in innate immune response against Gram-negative bacteria in macrophages, recognizing bacterial surface proteins and regulating phagosome maturation and PI3K complex II recruitment. Related references: PMID:20818396, PMID:22493499
• SLAMF1 is a single-pass transmembrane glycoprotein with a signal peptide, immunoglobulin domains, and disulfide bonds; it undergoes phosphorylation and is involved in cell adhesion and phagocytosis.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine optimal concentration for your cell type and staining conditions.
• Use appropriate isotype controls and Fc receptor blocking to ensure specific staining.
• Since the immunogen includes the extracellular domain (aa 25-242), this antibody is suitable for detecting surface CD150/SLAM on live, unfixed cells.
• Validate in mouse splenocytes, thymocytes, or activated T/B cells where CD150/SLAM is expressed.
• Protect the antibody from light during staining and storage to preserve the fluorophore.
• Fixation and permeabilization may affect the epitope; if needed, test compatibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your diagnostic and research workflows with a comprehensive portfolio of IVD raw materials. For immunology studies targeting CD150/SLAM, we offer a range of monoclonal antibodies, including this conjugate, complemented by ancillary reagents and custom sourcing. Our one-stop supply chain accelerates your development from concept to clinic.