Rabbit anti-Mouse CD146 Monoclonal Antibody for FC - Q8R2Y2

Core Product Specifications and Parameters

Biological Background: CD146 Function and Localization

• Cell surface glycoprotein MUC18 (also known as gicerin, MCAM) is a member of the immunoglobulin superfamily involved in cell adhesion.
• It contributes to the cohesion of the endothelial monolayer at intercellular junctions in vascular tissues.
• Expressed in melanoma cell lines, its presence may facilitate tumor cell interaction with the vascular system, aiding hematogenous metastasis.
• Acts as a receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, followed by a transient increase in intracellular calcium (by similarity).
• Subcellular localization: Membrane; single-pass type I membrane protein.
• Post-translational modifications: glycoprotein, disulfide bond, phosphoprotein.
• Plays a role in neural crest cell adhesion during embryonic development.
• Alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• This antibody is conjugated to , making it ready-to-use for flow cytometry without the need for secondary reagents.
• The immunogen corresponds to amino acids 24-563 of mouse CD146, covering the majority of the extracellular domain, suggesting recognition of surface-exposed epitopes.
• Validate the antibody's performance in the relevant mouse sample system, such as melanoma cell lines or endothelial cells, which are known to express CD146.
• For flow cytometry, titrate the antibody to determine the optimal staining concentration and include isotype controls to assess background staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined one-stop sourcing solution for IVD raw materials, from validated antibody pairs to bulk ancillary reagents. For projects involving CD146 and tumor adhesion biology, we provide monoclonal and polyclonal options, technical consultation, and rare target raw-material sourcing to support your workflow from development to commercialization.