Rabbit anti-Mouse CD146 Monoclonal Antibody for FC - Q8R2Y2

Core Product Specifications and Parameters

Biological Background: CD146 (MUC18) Function and Localization

• Cell surface glycoprotein MUC18, also known as CD146, melanoma cell adhesion molecule, or gicerin. It belongs to the immunoglobulin superfamily and is a single-pass type I membrane protein.
• Functions in cell adhesion, contributing to the cohesion of endothelial monolayers at intercellular junctions in vascular tissue. Its expression on melanoma cells may facilitate interactions with vascular elements, enhancing hematogenous tumor spread.
• During embryonic development, MUC18 may act as an adhesion molecule in neural crest cells.
• As a surface receptor, it triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and induces a transient increase in intracellular calcium concentration (by similarity).
• Predominantly localized to the cell membrane; detected in melanoma cell lines.
• Contains immunoglobulin-like domains, disulfide bonds, and is subject to N-glycosylation and phosphorylation. Alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen spans the majority of the extracellular domain (aa 24-563), ensuring recognition of native cell-surface CD146. This antibody is recommended for flow cytometry (FC) to detect mouse CD146 on live cells.
• Titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• As a rabbit monoclonal antibody, it offers high specificity and consistent lot-to-lot performance.
• The fluorophore provides bright red fluorescence, compatible with 561 nm or 594 nm lasers and standard PE/Cy5 filter sets.
• Cross-reactivity is validated for mouse; if testing other species, include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting, covering every stage from concept to clinic. This Rabbit anti-Mouse CD146 mAb supports research in cell adhesion, tumor metastasis, and vascular biology. As a trusted sourcing partner, we offer validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your development workflow.