Rabbit anti-Mouse CD144/VE-Cadherin Monoclonal Antibody for Flow Cytometry - P55284

Core Product Specifications and Parameters

Biological Background: Cadherin-5 Function and Localization

• Cadherin-5 (VE-cadherin) is a calcium-dependent cell adhesion protein encoded by the Cdh5 gene, belonging to the cadherin family of transmembrane glycoproteins.
• It mediates homophilic cell-cell adhesion in endothelial cells, crucial for maintaining intercellular junction cohesion and organization. Related references: PMID:20332120 PMID:9220534
• Through interactions with alpha-catenin, it links to the actin cytoskeleton, and couples actin fibers to cell junctions via scaffold proteins AMOTL2 and MAGI1. Related references: PMID:24806444
• Cadherin-5 collaborates with KRIT1 and PALS1 to establish endothelial cell polarity and vascular lumen formation. Related references: PMID:27466317
• It regulates cadherin endocytosis through association with CTNND1/p120-catenin, impacting junction dynamics. Related references: PMID:33972531
• Subcellularly, it localizes to adherens junctions and cell membrane, with cytoplasmic presence, and is expressed in postnatal endothelial cells, such as retinal vascular plexus.
• Post-translational modifications include phosphorylation and cleavage on basic residues; it is a metal-binding glycoprotein with a signal peptide and transmembrane helix.

Experimental Guidance and Technical Tips

• This antibody is validated for flow cytometry. For optimal results, consider titrating the antibody to determine the best concentration for your specific cell type and experimental conditions.
• Appropriate isotype controls and compensation controls are recommended when performing multicolor flow cytometry.
• Given the immunogen corresponds to the extracellular domain (aa 46-592), this antibody is likely suitable for detecting surface-expressed VE-cadherin without permeabilization. However, confirm with your sample.
• Validate the antibody in relevant mouse endothelial cell lines or primary cells, such as HUVEC equivalents or mouse retinal vascular endothelial cells, where CDH5 is expressed.

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