Rabbit anti-Mouse CD144/VE-Cadherin Monoclonal Antibody for FC - P55284

Core Product Specifications and Parameters

Biological Background: Cadherin-5 Function and Localization

• Cadherin-5, also known as VE-cadherin or CD144, is a classical cadherin that mediates calcium-dependent cell-cell adhesion through homophilic interactions.
• It plays a critical role in endothelial cell biology by controlling cohesion and organization of intercellular junctions, which is essential for vascular integrity. Related references: PMID:20332120 PMID:9220534
• Cadherin-5 associates with alpha-catenin, linking the junctional complex to the actin cytoskeleton, and anchors proteins such as AMOTL2 and MAGI1 to couple actin fibers to cell junctions. Related references: PMID:24806444
• In concert with KRIT1 and PALS1, it establishes and maintains endothelial cell polarity and vascular lumen formation by recruiting the Par polarity complex and RAP1B. Related references: PMID:27466317
• Cadherin-5 is involved in mechanotransduction, positively regulating reorientation of actin stress fibers and endothelial cell reorientation.
• It regulates its own endocytosis via association with CTNND1/p120-catenin, controlling junctional dynamics. Related references: PMID:33972531
• The protein localizes to adherens junctions, cell membrane, and cytoplasm, and is expressed in endothelial cells, including those of the retinal vascular plexus.
• Post-translational modifications include phosphorylation and cleavage on pairs of basic residues; it is a glycoprotein.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (aa 46-592), suggesting the antibody may recognize surface-exposed epitopes, making it suitable for flow cytometry applications.
• For flow cytometry using this directly conjugated antibody, consider titrating the antibody on target endothelial cells to determine optimal staining concentrations.
• Mouse endothelial cell lines or primary mouse endothelial cells are appropriate positive control samples; include appropriate isotype controls to ensure specificity.
• Always validate the antibody in your specific experimental system, as performance may vary with cell type and fixation/permeabilization protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw material procurement, providing validated monoclonal and polyclonal antibodies like this anti-CD144/VE-Cadherin conjugate to support vascular and endothelial research. We offer a comprehensive portfolio including antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, empowering diagnostic manufacturers from discovery to commercialization.