Rabbit anti-Mouse CD140b/PDGFR beta Monoclonal Antibody for Flow Cytometry - P05622

Core Product Specifications and Parameters

Biological Background: Platelet-derived Growth Factor Receptor beta Function and Localization

• Platelet-derived growth factor receptor beta (PDGFR beta, PDGFR-1, CD140b), encoded by the Pdgfrb gene in mouse, is a member of the type III receptor tyrosine kinase family characterized by immunoglobulin-like extracellular domains and a split intracellular kinase domain.
• It functions as a cell-surface receptor for homodimeric PDGFB and PDGFD, as well as heterodimers formed by PDGFA and PDGFB, playing an essential role in embryonic development, cell proliferation, survival, differentiation, chemotaxis, and migration.
• PDGFR beta is critical for blood vessel maturation, promoting the proliferation, migration, and recruitment of pericytes and vascular smooth muscle cells to endothelial cells, and is required for normal cardiovascular system development.
• In the kidney, it is necessary for the recruitment of mesangial cells into the glomerulus and for the formation of a branched capillary network; its expression is upregulated in renal fibrosis.
• Ligand binding activates multiple downstream signaling cascades, including PLCG1-mediated calcium mobilization and PKC activation, PI3K–AKT, and Ras–MAPK pathways, leading to cytoskeletal rearrangement and membrane ruffling.
• The receptor localizes primarily to the cell membrane but can be internalized into cytoplasmic vesicles and degraded in the lysosomal lumen following activation.
• Post-translational modifications include N-linked glycosylation, phosphorylation on tyrosine and serine/threonine residues, disulfide bond formation, and ubiquitination; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The antibody is directly conjugated to , requiring no secondary detection reagent for flow cytometry.
• For optimal staining, titrate the antibody (e.g., 0.25–2.0 µg per million cells) in the relevant cell type to determine the best signal-to-noise ratio.
• Use appropriate Fc receptor blocking and include viability dyes to exclude dead cells and reduce non-specific binding.
• The immunogen comprises the N-terminal extracellular domain (aa 32–530), suggesting reactivity with the ectodomain; fixation with paraformaldehyde may be compatible but should be validated.
• Validate PDGFR beta expression in your target population by including known positive controls (e.g., NIH/3T3 fibroblasts, vascular smooth muscle cells) and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, contract research organizations, and academic laboratories with a comprehensive range of IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare-target sourcing. For researchers investigating PDGFR beta in vascular biology, oncology, or fibrotic diseases, CamelBio can supply matched detection reagents, recombinant proteins, and technical consultation to streamline assay development and production scale-up. We bridge the gap from single-lab validation to clinical application, ensuring a reliable supply chain for your diagnostic and research needs.