Rabbit anti-Mouse CD14 Monoclonal Antibody for FC - P10810

Core Product Specifications and Parameters

Biological Background: CD14 Function and Localization

• CD14 is a monocyte differentiation antigen and a myeloid-cell-specific leucine-rich glycoprotein.
• It acts as a coreceptor for bacterial lipopolysaccharide (LPS): in concert with LBP, it binds monomeric LPS and delivers it to the LY96/TLR4 complex, initiating innate immune response. Related references: PMID:16148141
• LPS-activated CD14 signals through MyD88, TIRAP, and TRAF6, leading to NF-kappa-B activation, cytokine secretion, and inflammatory response. Related references: PMID:15895089, PMID:8612135
• It also serves as a coreceptor for TLR2:TLR6 and TLR2:TLR1 heterodimers in response to diacylated and triacylated lipopeptides, respectively, triggering signaling from the cell surface and subsequent targeting to the Golgi.
• CD14 binds electronegative LDL (LDL(-)) and mediates cytokine release induced by LDL(-).
• It functions as an accessory receptor for M. tuberculosis lipoproteins LprA, LprG, and LpqH, in conjunction with TLR2 and TLR1, modulating antigen-presenting cell functions. Related references: PMID:19362712
• Subcellularly, CD14 is expressed at the cell membrane, secreted into the extracellular space, and localized to membrane rafts and the Golgi apparatus.
• At the protein level, CD14 is detected on peritoneal macrophages and on lung alveolar, dendritic, and lung macrophages. Related references: PMID:8612135, PMID:19362712
• The protein features leucine-rich repeats, disulfide bonds, glycosylation, and a GPI-anchor; it exists as both a membrane-bound and a secreted form.

Experimental Guidance and Technical Tips

• For flow cytometry, consider titrating the antibody to determine optimal working concentration. Validate in the relevant mouse sample system, considering that CD14 is expressed on peritoneal and lung macrophages.
• The conjugate is compatible with standard blue laser (488 nm) detection and can be included in multicolor panels after compensation optimization.
• Given the GPI-anchored and secreted nature of CD14, ensure cell surface staining protocols include appropriate washing steps to minimize background from soluble receptor.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a centralized channel for IVD raw-material procurement, backed by technical and consulting services from concept to clinic. This anti-mouse CD14 antibody supports research in innate immunity, infectious disease, and macrophage biology. Complement your workflows with our validated antibody pairs, bulk ancillary reagents, and rare-target sourcing capabilities—streamlining development for flow cytometry and beyond.