Rabbit Anti-Mouse CD138/Syndecan-1 Monoclonal Antibody for IF-P and FC - P18828

Core Product Specifications and Parameters

Biological Background: Syndecan-1 Function and Localization

• Syndecan-1 (CD138) is a cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate glycosaminoglycan chains. Related references: PMID:8163535
• It functions as a linker between the cytoskeleton and the interstitial matrix, facilitating cell adhesion and signaling. Related references: PMID:8163535
• Syndecan-1 regulates exosome biogenesis through interactions with SDCBP (syntenin) and PDCD6IP (ALIX).
• In dental mesenchymal cells, Syndecan-1 can autoinduce its own expression via an MSX1-mediated pathway, influencing tissue morphogenesis. Related references: PMID:8898217
• The protein is found at the plasma membrane, is shed into the extracellular space, and is enriched in exosomes.
• Syndecan-1 undergoes post-translational modifications including phosphorylation and glycosylation, and alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 23-255), which is likely accessible in native conformations, supporting applications like flow cytometry and immunofluorescence.
• For IF-P, consider antigen retrieval methods to unmask the epitope in formalin-fixed paraffin-embedded tissues, and validate with appropriate mouse tissue controls.
• For flow cytometry, use standard cell surface staining protocols with live cells, and include an Fc block to reduce nonspecific binding.
• As the antibody is directly conjugated to , it can be used for multiplexing; verify spectral compatibility with other fluorophores.
• Cross-reactivity has been validated in mouse; when working with other species, perform sequence alignment and test experimentally.
• The predicted molecular weight is approximately 33 kDa, but glycosylation may cause a higher apparent molecular weight in western blot; note that this antibody is not validated for WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to validated antibody pairs, monoclonal antibodies like this anti-Syndecan-1 conjugate, and bulk ancillary reagents for exosome and cancer research. Our global supply network can source rare-target raw materials, supporting your IVD development from concept to clinic.