Rabbit anti-Mouse CD138/Syndecan-1 Monoclonal Antibody for Flow Cytometry - P18828

Core Product Specifications and Parameters

Biological Background: Syndecan-1 Function and Localization

• Syndecan-1 (CD138) is a cell surface proteoglycan that bridges the cytoskeleton to the interstitial matrix, containing both heparan sulfate and chondroitin sulfate chains. Related references: PMID:8163535
• It plays a role in exosome biogenesis through interactions with SDCBP and PDCD6IP, linking proteoglycan to the endosomal sorting machinery.
• Syndecan-1 can induce its own expression in mesenchymal and epithelial cells via an MSX1-mediated pathway, contributing to tissue morphogenesis. Related references: PMID:8898217
• The protein undergoes alternative splicing and is subject to post-translational modifications including phosphorylation, glycosylation, and attachment of heparan sulfate and chondroitin sulfate GAGs.
• Subcellularly, it localizes to the plasma membrane, is secreted into the extracellular space after shedding, and is also found in extracellular exosomes.
• Keywords: Alternative splicing, Glycoprotein, Heparan sulfate, Membrane, Phosphoprotein, Proteoglycan, Secreted, Signal, Transmembrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 23-255) of mouse Syndecan-1, favoring detection of the mature surface form; receptor shedding may reduce signal, so consider using protease inhibitors and rapid processing if staining for surface expression.
• As an directly conjugated antibody, it allows one-step flow cytometric detection, reducing background from secondary reagents— use appropriate isotype controls and Fc block for optimal signal-to-noise.
• Validate in mouse-derived samples; cross-reactivity with other species is not indicated, so test any non-mouse applications empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your single-source partner for IVD raw materials, supplying validated antibody pairs, optimized monoclonal and polyclonal antibodies, ancillary reagents, and rare target sourcing. This anti-CD138 antibody supports flow cytometry applications in exosome biology and matrix interaction research, with bulk and custom conjugation options available to streamline your diagnostic development pipeline. Let CamelBio bridge your concept-to-clinic journey with reliable, scalable sourcing solutions.