Rabbit anti-Mouse CD130/gp130 Monoclonal Antibody for Flow Cytometry - Q00560

Core Product Specifications and Parameters

Biological Background: gp130 (Interleukin-6 Receptor Subunit Beta) Function and Localization

• Interleukin-6 receptor subunit beta (IL6ST), also called gp130, CD130, or membrane glycoprotein 130, serves as the common signal-transducing receptor for the IL-6 cytokine family.
• Upon ligand binding, gp130 undergoes homodimerization (e.g., with IL-6/IL-11) or heterodimerization with LIFR/OSMR, leading to activation of associated JAK kinases, receptor phosphorylation, and recruitment/activation of STAT transcription factors. Negative feedback is exerted via induction of SOCS1 and SOCS3. Related references: PMID:10661409, PMID:1602143, PMID:9202125
• In addition to classical JAK/STAT signaling, gp130 activates YAP and NOTCH pathways to promote inflammation-induced epithelial regeneration independently of STAT3. Related references: PMID:25731159
• Gp130-mediated signals control a wide array of biological processes, including immune response, hematopoiesis, pain perception, bone metabolism and mass, embryonic development, and the survival of motor/sensory neurons and astrocyte differentiation. Related references: PMID:10661409, PMID:25057188, PMID:26255596, PMID:10377352, PMID:25228504, PMID:24339143, PMID:9348227
• Gp130 is broadly expressed in tissues such as brain, heart, thymus, spleen, kidney, lung, and liver, including specific neuronal populations like the paraventricular nucleus of the hypothalamus.
• The protein localizes to the cell membrane as a type I transmembrane glycoprotein and features immunoglobulin-like domains, disulfide bonds, and phosphorylation sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 23–617), suggesting the antibody recognizes cell-surface gp130.
• As a directly conjugated antibody, it requires no secondary detection reagent; protect from light and titrate to optimize resolution.
• Flow cytometry analysis of mouse gp130 should include appropriate isotype controls and consider the broad tissue expression pattern when validating in specific cell types.
• Shipping on ice bag; store according to manufacturer’s recommendations.

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