Rabbit anti-Mouse CD127/IL-7Rα Monoclonal Antibody for Flow Cytometry - P16872

Core Product Specifications and Parameters

Biological Background: CD127/IL-7Rα Function and Localization

• Interleukin-7 receptor subunit alpha (IL-7Rα), also known as CD127, is a type I transmembrane glycoprotein that functions as a specific receptor for interleukin-7 (IL-7) and thymic stromal lymphopoietin (TSLP).
• By binding IL-7, CD127 activates JAK/STAT, PI3K/Akt, and MAPK signaling cascades, which are essential for lymphocyte development, survival, and homeostasis.
• As a receptor for TSLP, CD127 contributes to the regulation of dendritic cell and T cell responses, influencing allergic inflammation and immune tolerance.
• CD127 is predominantly expressed on the surface of immune cells, particularly in the spleen, thymus, and fetal liver, consistent with its critical role in lymphopoiesis.
• The receptor localizes to the plasma membrane and contains an extracellular ligand-binding domain, a single transmembrane helix, and a cytoplasmic tail that mediates downstream signaling.
• Post-translational modifications include N-glycosylation, phosphorylation on multiple residues, and ubiquitination, which regulate receptor expression, trafficking, and signal transduction.

Experimental Guidance and Technical Tips

• This antibody targets an extracellular epitope (amino acids 21–239) of mouse CD127, making it directly compatible with live-cell staining for flow cytometry without permeabilization.
• The conjugate provides a bright signal with minimal spectral spillover; consider optimizing the antibody dilution by titration in the relevant experimental sample.
• Use freshly isolated splenocytes or thymocytes as positive control samples, as these tissues exhibit high CD127 expression, and include appropriate isotype controls.
• For dual or multicolor panels, verify fluorophore compatibility and adjust compensation settings as needed to avoid spectral overlap.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials, technical services, and consulting support—from concept to clinic. This Rabbit anti-Mouse CD127/IL-7Rα mAb is part of our comprehensive flow cytometry antibody collection, supporting immunological and cell signaling research. Contact us to explore validated antibody pairs, bulk ancillary reagents, and custom sourcing solutions tailored to your project needs.