Rabbit anti-Mouse CD127/IL-7Rα Monoclonal Antibody for Flow Cytometry - P16872

Core Product Specifications and Parameters

Biological Background: CD127/IL-7Rα Function and Localization

• Interleukin-7 receptor subunit alpha (IL-7Rα, CD127) is a type I transmembrane glycoprotein that serves as the specific alpha chain of the receptor for interleukin-7 (IL-7), and also functions as a receptor for thymic stromal lymphopoietin (TSLP).
• IL-7Rα is essential for lymphocyte development and homeostasis, mediating signaling that promotes survival, proliferation, and differentiation of T and B cell precursors.
• The receptor is expressed on the surface of various immune cells, with highest levels detected in the spleen, thymus, and fetal liver, reflecting its critical role in early lymphoid development.
• IL-7Rα is a single-pass membrane protein localized to the plasma membrane, where it associates with the common gamma chain (γc) to form the functional IL-7 receptor complex.
• The protein undergoes multiple post-translational modifications, including N-linked glycosylation, disulfide bond formation, and phosphorylation, which regulate its expression and signaling.
• IL-7Rα is also modified by ubiquitin conjugation (Ubl conjugation), suggesting involvement in receptor turnover and signal attenuation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain of mouse CD127 (aa 21-239), suggesting the antibody likely recognizes an epitope accessible on live cells for surface staining in flow cytometry.
• As a direct conjugate, this antibody eliminates the need for secondary detection, reducing background and simplifying multicolor panel design; typical starting concentrations for flow cytometry range from 0.5–5 µg/mL but require optimization.
• For optimal staining, consider using freshly isolated mouse splenocytes or thymocytes, where CD127 expression is expected; include appropriate isotype controls and viability dyes.
• Since CD127 expression can be modulated by activation or cytokines, validate the staining pattern under your specific experimental conditions.
• Avoid over-fixation with certain permeabilization protocols that may destroy the fluorophore or epitope; if intracellular staining is required, test compatibility first.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your strategic partner for IVD raw-material procurement, offering a comprehensive portfolio of validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents. This -conjugated anti-CD127 antibody supports your flow cytometry-based immunophenotyping and immune monitoring applications. With deep expertise in rare-target reagent sourcing and bulk supply, CamelBio bridges the gap from early R&D to diagnostic kit manufacturing.