Rabbit anti-Mouse CD127/IL-7Rα Monoclonal Antibody for FC - P16872

Core Product Specifications and Parameters

Biological Background: IL-7Rα Function and Localization

• CD127, also known as IL-7Rα, is the alpha subunit of the interleukin-7 receptor, a type I cytokine receptor family member.
• It functions as the receptor for interleukin-7 (IL-7), a cytokine essential for lymphocyte development and homeostasis.
• CD127 also serves as a receptor for thymic stromal lymphopoietin (TSLP), implicating it in allergic inflammation and T cell responses.
• The receptor is a single-pass transmembrane glycoprotein located on the cell membrane.
• It is expressed in lymphoid tissues including spleen, thymus, and fetal liver.
• Post-translational modifications include glycosylation and phosphorylation, and the extracellular domain contains disulfide bonds critical for structure.
• The predicted molecular weight of mouse IL-7Rα is approximately 51–52 kDa, consistent with the reported 51 kDa.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant fusion protein covering the extracellular domain (amino acids 21–239) of mouse CD127, which contains the ligand-binding region. This design aids in generating antibodies that recognize the native surface receptor.
• This antibody is validated for flow cytometry (FC). When performing FC, consider optimizing the antibody dilution and including appropriate Fc blocking to minimize non-specific binding.
• As an conjugate, ensure your flow cytometer has the appropriate laser and filter configuration (typically 633–647 nm excitation) for detection.
• Validate specificity and sensitivity in the relevant mouse cell lines or primary cells known to express CD127 (e.g., T cells, B cell progenitors).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive B2B sourcing partner for IVD raw materials, offering validated antibody pairs, recombinant proteins, and ancillary reagents to support diagnostic development. For researchers and developers working on interleukin-7 receptor biology and immunophenotyping, CamelBio streamlines procurement with reliable monoclonal antibodies like this conjugate. Contact our team to explore customized sourcing and technical support for your next immunology project.