Rabbit Anti-Mouse CD126/IL-6Rα chain Monoclonal Antibody for FC - P22272

Core Product Specifications and Parameters

Biological Background: IL-6 Receptor Subunit Alpha Function and Localization

• Interleukin-6 receptor subunit alpha (IL-6Rα), also designated CD126, IL-6R, or IL-6RA, is a single-pass type I membrane protein belonging to the immunoglobulin superfamily. It functions as the ligand-binding component of the IL-6 receptor complex, which is essential for IL-6 signal transduction.
• The membrane-bound IL-6Rα binds IL-6 with low affinity and must associate with the signal-transducing subunit IL6ST/gp130 to initiate intracellular signaling. This classic signaling is restricted to cells expressing IL-6Rα, such as hepatocytes and certain immune cells, and is generally associated with regenerative and anti-inflammatory outcomes. Cluster signaling occurs when membrane-bound IL-6/IL-6Rα complexes on one cell activate IL6ST on adjacent cells.
• IL-6Rα signaling directs naive CD4+ T cells toward the Th17 lineage through cluster signaling mediated by dendritic cells. Related references: PMID:27893700
• Soluble IL-6Rα (sIL-6R), generated by proteolytic cleavage or alternative splicing, acts as an agonist of IL-6. The IL-6:sIL-6R complex can stimulate cells that do not express membrane-bound IL-6Rα through trans-signaling, driving pro-inflammatory processes and contributing to chronic inflammatory diseases. Related references: PMID:11113088
• IL-6 trans-signaling via sIL-6R is required for liver tissue regeneration and protection during injury. Related references: PMID:11113088
• In the central nervous system, IL-6 trans-signaling regulates energy and glucose homeostasis. Related references: PMID:28402851
• IL-6Rα is localized to the cell membrane and is also secreted. It is expressed by dendritic cells and has been detected in cerebrospinal fluid.
• The protein is a glycoprotein containing immunoglobulin-like domains and disulfide bonds, with a calculated molecular weight of about 50 kDa.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 20–357 of the mouse IL-6Rα extracellular domain, which includes the ligand-binding region. This design promotes the generation of antibodies that recognize the native receptor on live cells.
• For flow cytometry using the conjugate, determine the optimal staining concentration by titration on cells known to express IL-6Rα (e.g., activated T cells, dendritic cells, or hepatocyte lines). Include appropriate isotype and fluorescence-minus-one controls.
• has excitation/emission peaks similar to Alexa Fluor® 594; avoid spectral overlap with PE, Texas Red, and other red fluorophores. Ensure proper compensation using single-stained controls.
• Assess cell viability before staining, as dead cells may bind antibodies nonspecifically. Fixation and permeabilization are not recommended for surface antigens unless validated.
• For blocking, use 5–10% normal serum from the host species of the secondary antibody if indirect staining, but direct conjugate may require minimal blocking. Validate in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers IVD manufacturers and research institutions with comprehensive access to premium raw materials and technical support from discovery to commercialization. This anti-IL-6Rα monoclonal antibody, pre-conjugated to , streamlines flow cytometry workflows for immune signaling and inflammation studies. Beyond single reagents, we supply validated antibody pairs, bulk ancillary proteins, and custom sourcing for rare targets—enabling you to accelerate assay development with confidence.