Rabbit anti-Mouse CD126/IL-6Rα chain mAb - P22272

Core Product Specifications and Parameters

Biological Background: IL-6Rα Function and Localization

• IL-6Rα (Interleukin-6 receptor subunit alpha) is a type I transmembrane glycoprotein belonging to the type I cytokine receptor family, featuring an extracellular region with immunoglobulin-like domains and a conserved WSXWS motif.
• It serves as the low-affinity binding subunit for IL-6 and cannot signal independently; the formation of the high-affinity complex with IL6ST/gp130 is required to activate downstream JAK/STAT and MAPK pathways.
• Classical IL-6 signaling, mediated by membrane-bound IL-6Rα, is predominantly regenerative and anti-inflammatory, and its expression is largely restricted to hepatocytes and certain immune cell subsets.
• Soluble IL-6Rα (sIL6R), produced by proteolytic cleavage or alternative splicing, complexes with IL-6 to enable trans-signaling via gp130 on cells lacking endogenous IL-6Rα. This pro-inflammatory mode is central to chronic inflammatory conditions (PubMed:11113088; By similarity). Related references: PMID:11113088
• Through cluster signaling, membrane-bound IL-6/IL-6Rα complexes on dendritic cells drive the polarization of naive CD4+ T cells toward a Th17 phenotype, linking innate and adaptive immunity (PubMed:27893700). Related references: PMID:27893700
• sIL6R is required for hepatoprotection and liver regeneration following injury (PubMed:11113088), and trans-signaling in the central nervous system is critical for the regulation of energy and glucose homeostasis (PubMed:28402851). Related references: PMID:11113088 PMID:28402851
• The protein is modified by N‑linked glycosylation and contains conserved disulfide bonds, which are essential for proper folding, stability, and ligand interaction.
• IL-6Rα is expressed on dendritic cells in lymphoid tissues and is found as a soluble form in the cerebrospinal fluid, reflecting its dual roles in immune surveillance and neuroimmune crosstalk.

Experimental Guidance and Technical Tips

• The immunogen encompasses the entire extracellular region (aa 20‑357), so the antibody likely detects an epitope within the ligand‑binding domain and may recognize both membrane‑bound and soluble forms of IL‑6Rα.
• For flow cytometry, titrate the antibody to determine the optimal staining concentration; use isotype controls and Fc receptor blocking to minimize background.
• Ensure that your flow cytometer is equipped with an appropriate laser (e.g., 633‑647 nm) and filter (e.g., 660/20) for the fluorophore.
• Validate the antibody in relevant mouse cell populations, such as splenocytes or bone marrow‑derived dendritic cells, which are known to express IL‑6Rα.
• Consider that the antibody may neutralize or block IL‑6 binding if its epitope overlaps with the cytokine‑binding site; pre‑incubation with recombinant IL‑6 can help determine if staining is affected.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated provider of high‑quality IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. This anti‑mouse IL‑6Rα antibody supports research into immune regulation, inflammation, and cytokine signaling pathways. Leverage our one‑stop sourcing solutions—from bulk monoclonal antibodies to custom assay development—to accelerate your diagnostic and research programs.