Rabbit anti-Mouse CD124/IL-4Ra Monoclonal Antibody for FC - P16382

Core Product Specifications and Parameters

Biological Background: CD124/IL-4Ra Function and Localization

• CD124, also known as interleukin-4 receptor subunit alpha (IL-4Rα), is the ligand-binding component of the type I and type II IL-4 receptors, mediating responses to both interleukin 4 (IL-4) and interleukin 13 (IL-13).
• Upon ligand binding, IL-4Rα couples to the JAK1/2/3-STAT6 signaling pathway, playing a central role in T-helper 2 (Th2) cell differentiation and the regulation of IgE production, chemokine secretion, and mucus production at sites of allergic inflammation.
• In certain cell types, IL-4Rα can also signal through activation of insulin receptor substrates IRS1 and IRS2.
• The receptor is predominantly located at the cell membrane, but a secreted soluble form can be generated through alternative splicing.
• CD124 is a glycoprotein and phosphoprotein, containing disulfide bonds and a single-pass transmembrane helix.
• It is expressed in both Th1 and Th2 cells, indicating its broad role in T-cell biology beyond Th2 responses.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 26-233 of mouse CD124, which represents the extracellular domain; thus the antibody is expected to recognize the extracellular portion of the receptor.
• This antibody is directly conjugated to , making it ready for flow cytometry (FC) applications without the need for secondary staining.
• Consider titrating the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• Include appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding in flow cytometry experiments.
• Validate the staining in relevant mouse cell lines or primary cells known to express CD124, such as T lymphocytes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers investigating IL-4/IL-13 signaling and allergic inflammation, CamelBio offers a reliable supply of validated antibodies, including this Rabbit anti-Mouse CD124/IL-4Ra mAb, alongside a full portfolio of ancillary reagents and bulk raw materials. As a sourcing partner, we specialize in bridging supply gaps for rare targets and customized immunoreagents, ensuring your projects stay on track with seamless procurement and expert support.