Rabbit anti-Mouse CD124/IL-4Ra mAb for FC - P16382

Core Product Specifications and Parameters

Biological Background: CD124/IL-4Ra Function and Localization

• Interleukin-4 receptor subunit alpha (IL-4Rα), encoded by the Il4r gene, is a transmembrane receptor that also serves as a subunit of the IL-13 receptor complex, binding both IL-4 and IL-13 cytokines.
• Upon ligand binding, IL-4Rα couples to the JAK1/2/3-STAT6 signaling pathway, leading to transcriptional regulation of genes involved in immune responses, notably promoting T helper 2 (Th2) cell differentiation.
• IL-4Rα signaling regulates immunoglobulin E (IgE) production, as well as chemokine and mucus production at sites of allergic inflammation, thereby playing a key role in allergic responses.
• In certain cell types, IL-4Rα can also signal through activation of insulin receptor substrates (IRS1/IRS2), linking immune signaling to metabolic pathways.
• The receptor is expressed on the surface of both Th1 and Th2 cells (cell membrane) and can also be found in a secreted form, as indicated by alternative splicing.
• As a glycoprotein with disulfide bonds and phosphorylation sites, IL-4Rα undergoes post-translational modifications that regulate its stability and signaling activity.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to the extracellular domain (amino acids 26-233) of mouse IL-4Rα, suggesting the antibody likely recognizes an epitope accessible on the cell surface, making it suitable for flow cytometry applications.
• For flow cytometry (FC) experiments, titration of the antibody and appropriate isotype controls are recommended to optimize staining and minimize background.
• As the target is a cell surface receptor, consider using freshly isolated cells or optimized fixation protocols to preserve epitope integrity; avoid permeabilization unless specifically targeting intracellular pools.
• Cross-reactivity is reported for mouse only; thus, verify reactivity in the relevant mouse strain or cell line system before use in other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes by offering a consolidated supply of high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers studying IL-4/IL-13 signaling or allergic inflammation, this anti-CD124 antibody facilitates reliable flow cytometric detection. CamelBio extends support with rare target raw-material sourcing and end-to-end technical consultation, streamlining your path from concept to clinic.