Rabbit anti-Mouse CD123 Monoclonal Antibody for FC - P26952

Core Product Specifications and Parameters

Biological Background: CD123 (Interleukin-3 receptor alpha) Function and Localization

• CD123, also known as Interleukin-3 receptor alpha chain (IL-3Rα), is encoded by the Il3ra gene (Sut-1) and serves as the ligand-binding subunit of the IL-3 receptor complex. Alternative splicing produces multiple isoforms.
• It is expressed on the surface of hematopoietic progenitor cells, monocytes, and B-lymphocytes, where it controls the production and differentiation of these cells into lineage-restricted populations.
• Ligand binding induces heterodimerization with the common beta chain (IL3RB), leading to activation of JAK2 and PI3K signaling pathways, which promote cell proliferation and differentiation. Related references: PMID:10477686, PMID:31990690
• Activation of JAK2 triggers STAT5-mediated transcriptional programs essential for hematopoietic cell survival and proliferation. Related references: PMID:10376805
• The receptor is localized at the cell membrane and throughout the endomembrane system, reflecting its trafficking as a transmembrane glycoprotein.
• Post-translational modifications include glycosylation, disulfide bond formation, and ubiquitin conjugation (isopeptide bonds), which may regulate receptor stability and signaling.
• The protein has a calculated molecular weight of approximately 43 kDa (43195 Da).

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (amino acids 17-331) of mouse CD123, which likely targets the native receptor on the cell surface. This design may help in detecting both the unbound and IL-3-bound conformations.
• For flow cytometry (FC), titrate the antibody to determine the optimal staining concentration. Use appropriate isotype controls to assess non-specific binding.
• As the antibody is specific for mouse CD123, it is not expected to cross-react with human or other species. If cross-reactivity is desired, validate experimentally.
• Consider testing alternative splicing isoforms, as the antibody's epitope may be affected by isoform-specific sequence variations.
• The conjugate allows direct detection without a secondary antibody; avoid unnecessary freeze-thaw cycles to maintain conjugate stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including this Rabbit anti-Mouse CD123 mAb for flow cytometric analysis of hematopoietic cells. As a one-stop sourcing partner, CamelBio provides validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to support your diagnostic development from concept to clinic.