Rabbit anti-Mouse CD11b Monoclonal Antibody for Flow Cytometry - P05555

Core Product Specifications and Parameters

Biological Background: Integrin alpha-M Function and Localization

• Integrin alpha-M (ITGAM), also known as CD11b, CR3, and MAC-1, is the alpha subunit of the leukocyte integrin heterodimer ITGAM/ITGB2 (CD11b/CD18), a cell surface receptor belonging to the integrin family.
• The ITGAM/ITGB2 complex functions as a receptor for the iC3b fragment of complement component C3, fibrinogen, and factor X. It mediates adhesive interactions of monocytes, macrophages, and granulocytes, and is critical for the uptake of complement-coated particles and pathogens.
• This integrin recognizes the R-G-D peptide in C3b and binds to P1 and P2 peptides of the fibrinogen gamma chain. It mediates neutrophil migration and, together with ICAM1, ensures adhesion between stimulated neutrophils and endothelial cells.
• In microglia, ITGAM partners with TYROBP/DAP12 to regulate the production of superoxide ions, which promote neuronal apoptosis during brain development. Related references: PMID:18685038
• Subcellularly, integrin alpha-M localizes to the cell membrane and is enriched in membrane rafts, where it facilitates rapid signal transduction.
• Predominantly expressed in monocytes and granulocytes, with additional expression in a subset of peritoneal mast cells and in microglia.
• Post-translational modifications include glycosylation and disulfide bond formation; the protein contains calcium- and metal-binding domains, undergoes alternative splicing, and features a signal peptide and a single-pass transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 143–337 of the mouse CD11b extracellular region; this region is likely exposed in the native conformation, making the antibody suitable for detecting cell surface CD11b by flow cytometry.
• For flow cytometry, titrate the antibody to determine the optimal working concentration and include appropriate isotype controls. Validate staining using mouse monocyte/macrophage cell lines or primary cells such as peritoneal macrophages or bone marrow-derived monocytes.
• Since CD11b is expressed on granulocytes and microglia, consider using this antibody for immunophenotyping of myeloid cells and for studies involving complement-mediated phagocytosis.

CamelBio: Your One-Stop Sourcing Bridge

• As a comprehensive IVD raw-material supplier, CamelBio streamlines the procurement of high-specificity antibodies like this CD11b clone, supporting diagnostic manufacturers and research teams in immunology and inflammation studies. Beyond monoclonal antibodies, we offer validated antibody pairs, recombinant proteins, and ancillary reagents to accelerate assay development from concept to clinical application.