Rabbit anti-Mouse CD11b Monoclonal Antibody for Flow Cytometry - P05555

Core Product Specifications and Parameters

Biological Background: Integrin alpha-M Function and Localization

• Integrin alpha-M (CD11b) is a cell adhesion receptor that pairs with integrin beta-2 (CD18) to form the Mac-1 (CR3) complex, primarily expressed on myeloid leukocytes.
• Mediates leukocyte adhesion, migration, and phagocytosis by binding ligands including iC3b, fibrinogen, factor X, and ICAM1 on endothelial cells.
• Functions as the complement receptor 3 (CR-3), recognizing the iC3b fragment of complement component C3, facilitating opsonized pathogen clearance.
• Located on the cell membrane and enriched in membrane rafts, contributing to signal transduction during immune responses.
• Expressed at high levels in monocytes, granulocytes, and subsets of peritoneal mast cells, and is critical for microglial function in the brain. Related references: PMID:3887182 PMID:8986723 PMID:9862668 PMID:18685038
• In microglia, ITGAM/ITGB2 collaborates with TYROBP to regulate superoxide production, influencing neuronal apoptosis during brain development. Related references: PMID:18685038
• The protein is heavily glycosylated and contains disulfide bonds, with multiple metal-binding sites, classifying it as a calcium-dependent integrin.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 143–337, a region within the extracellular ligand-binding domain; consider this for applications requiring native epitope recognition.
• This conjugate is designed for flow cytometry; titrate the antibody to determine the optimal staining concentration for your specific mouse cell type.
• Use appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding, especially when analyzing granulocyte or macrophage populations.
• For detection of surface CD11b, avoid permeabilization unless intracellular staining is also required; validate fixation conditions (e.g., 2–4% PFA) to preserve epitope integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with reliable sourcing of IVD raw materials, including validated monoclonal antibodies like this anti-mouse CD11b flow cytometry reagent. Our portfolio spans optimized antibody pairs, bulk ancillary reagents, and custom procurement for rare targets involved in immunology and cell adhesion research. Whether you need to scale up for assay development or secure consistent batch-to-batch performance, CamelBio serves as your dedicated B2B partner from concept to clinic.