Rabbit anti-Mouse CD11b Monoclonal Antibody () for Flow Cytometry - P05555

Core Product Specifications and Parameters

Biological Background: Integrin alpha-M Function and Localization

• Integrin alpha-M (CD11b) is a leukocyte-specific integrin that pairs with integrin beta-2 (CD18) to form the Mac-1 heterodimer, also known as complement receptor 3 (CR3).
• It facilitates adhesive interactions of monocytes, macrophages, and granulocytes, mediating the uptake of complement-coated particles and pathogens by binding the iC3b fragment of complement component C3.
• ITGAM/ITGB2 also serves as a receptor for fibrinogen and factor X, recognizing specific peptide sequences in the fibrinogen gamma chain, contributing to coagulation and inflammatory responses.
• The protein is predominantly expressed in monocytes, granulocytes, and microglia, with expression also observed in subsets of peritoneal mast cells.
• In microglia, ITGAM/ITGB2 is required with TYROBP/DAP12 for producing superoxide ions that promote neuronal apoptosis during brain development. Related references: PMID:18685038
• Subcellularly, CD11b localizes to the cell membrane and membrane rafts, where it participates in signaling and adhesion events.
• The protein contains calcium-binding domains, is heavily glycosylated, and features multiple disulfide bonds, characteristic of integrin alpha chains.
• Alternative splicing generates multiple isoforms, expanding the functional repertoire of this adhesion molecule.

Experimental Guidance and Technical Tips

• This -conjugated antibody is optimized for direct flow cytometry applications; titrate the antibody to determine the optimal staining concentration for your specific cell type and protocol.
• Include appropriate isotype controls and live/dead or viability staining to ensure accurate gating and analysis.
• Validate staining specificity in relevant mouse tissue or cell samples, such as monocytes, granulocytes, or microglia, under appropriate fixation and permeabilization conditions if needed.
• The fluorophore requires excitation with a red laser (633–647 nm) and is detectable in the far-red channel; ensure instrument compatibility and compensation setup.
• For multicolor panels, confirm spectral compatibility with other fluorophores and perform single-stain compensation controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials, including validated antibodies like this anti-CD11b clone. Our sourcing network supports bulk orders of conjugates, antibody pairs, and ancillary reagents tailored for flow cytometry and immunoassay development. For integrin biology and leukocyte adhesion research, CamelBio can also assist with rare target sourcing and custom production to bridge the gap from concept to clinical application.