Rabbit anti-Mouse CD11b Monoclonal Antibody for FC - P05555

Core Product Specifications and Parameters

Biological Background: CD11b (Integrin alpha-M) Function and Localization

• ITGAM (CD11b) is the integrin alpha-M chain that pairs with integrin beta-2 (ITGB2/CD18) to form the heterodimeric receptor Mac-1 (CR3), a key leukocyte adhesion molecule.
• It mediates diverse adhesive interactions of monocytes, macrophages, and granulocytes, including binding to complement fragment iC3b (via R-G-D motif), fibrinogen, and factor X.
• Acts as a receptor for ICAM1, enabling adhesion between stimulated neutrophils and endothelial cells, and regulates neutrophil migration.
• In microglia, ITGAM cooperates with TYROBP/DAP12 to control superoxide ion production, which promotes neuronal apoptosis during brain development. Related references: PMID:18685038
• Subcellular localization includes the cell membrane and membrane rafts, consistent with its signaling and adhesion roles.
• Predominantly expressed in monocytes and granulocytes, with additional expression in a subset of peritoneal mast cells and in microglia at the protein level.
• Contains disulfide bonds, multiple calcium-binding sites, and a signal peptide; undergoes alternative splicing and is predicted to be glycosylated.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to ; users may perform cell surface staining without a secondary antibody. Titrate the antibody to determine the optimal concentration for the specific cell type and application.
• CD11b is highly expressed on myeloid cells; validate staining in relevant mouse cell lines (e.g., RAW 264.7 macrophages) or primary leukocytes from blood, spleen, or bone marrow.
• For multicolor flow cytometry panels, include appropriate single-stain controls and compensation controls to correct for spectral overlap, especially with other far-red fluorophores.
• If intracellular domains are of interest, consider that the immunogen spans amino acids 143–337, an extracellular region; permeabilization protocols should be optimized.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to the full breadth of IVD raw materials. For projects involving leukocyte biology, inflammation, or neuroimmunology, we supply ready-to-use validated antibody pairs, conjugated monoclonal antibodies like this anti-CD11b clone, and bulk ancillary reagents. Our sourcing network also supports hard-to-find targets and custom development, ensuring you move from concept to clinic with a single partner.