Rabbit anti-Mouse CD11a Monoclonal Antibody for Flow Cytometry - P24063

Core Product Specifications and Parameters

Biological Background: CD11a Function and Localization

• CD11a pairs with integrin beta-2 (ITGB2) to form LFA-1, a receptor for multiple ICAM proteins (ICAM1-4), facilitating leukocyte adhesion and immune synapse formation. Related references: PMID:2051027
• LFA-1 also acts as a receptor for the secreted form of ISG15, mediating a novel ubiquitin-like signaling pathway in immune cells. Related references: PMID:29100055
• It is critical for leukocyte-endothelial interactions, enabling T-cell and neutrophil transmigration during inflammation. Related references: PMID:16234355, PMID:24158516
• CD11a plays a role in lymphopoiesis by supporting the generation of common lymphoid progenitor cells in bone marrow. Related references: PMID:25108025
• This protein is predominantly expressed on all leukocytes and localizes to the cell membrane, specifically enriched in membrane rafts.
• Additional features include alternative splicing, disulfide bond-dependent structure, and multiple metal-binding sites (calcium, magnesium) essential for ligand binding.
• Post-translational modifications, such as glycosylation and phosphorylation, regulate its adhesive activity and signal transduction.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 149–340) of mouse CD11a, which includes part of the ligand-binding domain, making it suitable for detecting native surface protein.
• This antibody is conjugated with , a bright fluorophore optimized for flow cytometry (FC). It is recommended to titrate the antibody to determine the optimal staining concentration for your specific cell type.
• Cross-reactivity is confirmed for mouse; potential reactivity with other species should be tested if required.
• The predicted molecular weight is 128 kDa; confirm band identity using appropriate molecular weight markers in any supporting validation experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for the IVD industry, connecting diagnostic manufacturers and research teams with high-quality raw materials like this anti-mouse CD11a antibody. For immunology and cell adhesion research, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development from concept to clinic.