Rabbit anti-Mouse CD11a Monoclonal Antibody for Flow Cytometry - P24063

Core Product Specifications and Parameters

Biological Background: Integrin alpha-L Function and Localization

• Integrin alpha-L (CD11a) pairs with integrin beta-2 (CD18) to form the LFA-1 complex, a key leukocyte adhesion receptor that binds to ICAM1, ICAM2, ICAM3, and ICAM4. Related references: PMID:2051027
• LFA-1 mediates leukocyte adhesion and transmigration across endothelium, contributing to T-cell and neutrophil recruitment. Related references: PMID:16234355 PMID:24158516
• The integrin is critical for natural killer cell cytotoxicity and cytotoxic T-cell-mediated killing.
• LFA-1 acts as a signaling receptor for ISG15, linking innate immunity to leukocyte adhesion. Related references: PMID:29100055
• It plays an essential role in lymphopoiesis, being required for the generation of common lymphoid progenitor cells in bone marrow. Related references: PMID:25108025
• Subcellularly, CD11a is localized to the cell membrane and membrane rafts, where it participates in immunological synapse formation.
• Tissue expression is restricted to leukocytes, consistent with its role in immune cell function.
• Post-translational modifications include glycosylation, disulfide bonds, and phosphorylation; calcium and magnesium binding are essential for integrin conformational activation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an extracellular domain (amino acids 149–340), making this antibody suitable for detecting surface CD11a in flow cytometry.
• Because CD11a is expressed on leukocytes, validate the antibody on mouse splenocytes or peripheral blood mononuclear cells.
• Titrate the antibody to determine the optimal staining index for your flow cytometry assay; typical starting concentrations range from 0.1–1 µg per million cells.
• For multiplex panels, ensure minimal spectral overlap with other fluorophores when using the conjugate.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we streamline IVD raw material procurement by offering a wide range of high-quality antibodies and reagents. From validated antibody pairs to bulk ancillary reagents, we support diagnostic manufacturers and researchers in immunology and leukocyte biology. Explore our portfolio for reliable monoclonal antibodies like this CD11a clone to accelerate your assay development.