Rabbit anti-Mouse CD119/IFN-gamma receptor 1 mAb - P15261

Core Product Specifications and Parameters

Biological Background: IFNGR1 (CD119) Function and Localization

• IFNGR1, also known as CD119 or IFN-gamma receptor alpha-chain, is the ligand-binding subunit of the interferon gamma receptor complex.
• It mediates crucial antimicrobial, antiviral, and antitumor immune responses by activating effector cells and enhancing antigen presentation. Related references: PMID:20926559, PMID:27286456
• Functional receptor formation requires association with the accessory factor IFNGR2. Related references: PMID:2137461, PMID:2530216, PMID:2530582
• Upon IFN-gamma binding, the intracellular domain undergoes conformational change, allowing JAK1/JAK2 recruitment. JAK1 phosphorylates IFNGR1, creating a docking site for STAT1, which then dimerizes, translocates to the nucleus, and activates transcription of IFN-gamma-responsive genes. Related references: PMID:19889125
• STAT3 can also be activated through IFNGR1, although to a lesser extent. Related references: PMID:15284232
• The JAK-STAT pathway is regulated by SOCS1, which competes with STAT1 for binding to phosphorylated IFNGR1. Related references: PMID:15522878
• IFNGR1 is a cell membrane glycoprotein with an extracellular immunoglobulin-like domain, disulfide bonds, and intracellular phosphorylation and ubiquitination sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 26–250), suggesting the antibody targets an extracellular epitope. This makes it suitable for surface staining of live cells in flow cytometry.
• is a bright fluorophore; titrate the antibody to determine the optimal concentration for your cell type and experimental conditions to minimize background and maximize signal-to-noise ratio.
• Use appropriate isotype controls and Fc receptor blocking reagents to confirm binding specificity in mouse cells.
• Since IFNGR1 is expressed on various immune cells (e.g., T cells, macrophages), validate staining in relevant positive and negative cell populations.
• For intracellular staining or simultaneous detection of phosphorylated STAT1, fixation and permeabilization protocols may be optimized separately, as the antibody targets a surface receptor.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic developers from concept to clinical application. For interferon gamma signaling research, CamelBio offers a range of validated monoclonal antibodies, including conjugated formats like this anti-IFNGR1, along with matched antibody pairs and bulk ancillary reagents. Our expertise also extends to sourcing rare-target raw materials, providing tailored solutions to accelerate your assay development.