Rabbit anti-Mouse CD117/c-Kit Monoclonal Antibody for Flow Cytometry - P05532

Core Product Specifications and Parameters

Biological Background: CD117/c-Kit Function and Localization

• CD117/c-Kit is the receptor for stem cell factor (KITLG/SCF) and belongs to the type III receptor tyrosine kinase family, encoded by the Kit proto-oncogene.
• Upon ligand binding, it activates multiple downstream signaling cascades, including PI3K/AKT, RAS/MAPK, and JAK/STAT pathways, regulating cell survival, proliferation, and differentiation.
• Key physiological roles include hematopoiesis, stem cell maintenance, gametogenesis, mast cell development and function, and melanogenesis.
• The protein exists in several isoforms generated by alternative splicing; isoform 1 is the canonical full-length transmembrane receptor widely expressed in fetal liver and bone marrow.
• Subcellular localization includes the cell membrane and cytoplasm; internalization and degradation of the receptor modulate signaling.
• Post-translational modifications include glycosylation, disulfide bonds, and autophosphorylation on tyrosine residues, essential for kinase activation and downstream signaling.
• The UniProt keyword annotation classifies it as a glycoprotein, kinase, transmembrane protein, and proto-oncogene, highlighting its structural and functional complexity.

Experimental Guidance and Technical Tips

• This antibody is generated against a recombinant protein spanning amino acids 25-523, which corresponds to the extracellular domain of mouse CD117/c-Kit. It is ideal for flow cytometry applications to detect surface expression.
• For optimal results, use standard staining protocols with appropriate isotype controls. Avoid fixation/permeabilization if targeting the extracellular domain unless validated.
• Validate the antibody in your experimental system, particularly if analyzing rare cell populations like hematopoietic stem cells or mast cells.
• Since the antibody is directly conjugated to , no secondary antibody is needed, reducing background and simplifying the workflow.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio, your one-stop IVD raw-material sourcing partner, provides high-quality antibodies like this anti-CD117/c-Kit monoclonal antibody to support hematopoietic and oncology research. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your diagnostic development pipeline.