Rabbit anti-Mouse CD117/c-Kit Monoclonal Antibody for Flow Cytometry - P05532

Core Product Specifications and Parameters

Biological Background: CD117/c-Kit Function and Localization

• CD117/c-Kit is a tyrosine-protein kinase receptor for stem cell factor (SCF/KITLG), encoded by the Kit gene. Multiple isoforms exhibit distinct tissue distributions.
• Upon SCF binding, KIT activates downstream signaling cascades including PI3K/AKT, RAS/MAPK, and JAK/STAT pathways, which regulate cell survival, proliferation, hematopoiesis, and gametogenesis.
• The receptor structure includes five extracellular immunoglobulin-like domains, a single transmembrane helix, and an intracellular tyrosine kinase domain.
• KIT is primarily a cell membrane receptor; it undergoes internalization upon activation and can transiently localize to the cytoplasm.
• Autophosphorylation on tyrosine residues triggers downstream signaling, while ubiquitination regulates receptor degradation and signal attenuation.
• Isoforms 1 and 2 are expressed in bone marrow, spermatogonia, and spermatocytes; isoform 3 is detected in later-stage germ cells. Isoform 1 is widely expressed in hematopoietic tissues.
• As a proto-oncogene, KIT is associated with oncogenic processes when dysregulated; its mutations or overexpression are linked to various malignancies.

Experimental Guidance and Technical Tips

• This conjugate is validated for direct flow cytometry on mouse samples. For other applications (e.g., IHC, WB), optimization and independent validation are recommended.
• The immunogen corresponds to the extracellular domain (aa 25–523), making the antibody suitable for surface staining of live cells without permeabilization.
• Excitation and detection: can be excited by a red laser (633–640 nm) and detected in the APC channel. Ensure proper compensation in multicolor panels.
• To improve live/dead discrimination, consider co-staining with a viability dye, as CD117 may be shed or internalized in apoptotic cells.
• For quantitative flow cytometry, include an appropriate isotype control and perform antibody titration to determine optimal staining concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop partner for IVD raw-material sourcing, offering reliable access to validated monoclonal antibodies like this CD117/c-Kit clone. Our supply platform supports diagnostic manufacturers and researchers with bulk ancillary reagents, custom rare-target procurement, and validated antibody pairs, ensuring consistent, quality-assured materials for hematopoietic stem cell and oncology applications.